We continue the effort of monitoring diamondback moth (DBM) across the Salinas Valley using sex pheromone baited traps, as shown in the picture above. We have been adding additional traps to cover a larger area along Highway 101. We have daily moth capture data from Castroville to Greenfield. The chart below summarizes these daily captures from our pheromone traps.

As stated in a previous blog post, higher numbers of DBM have been usually recorded in Castroville. It seems like we just passed a generation of adults during late March to early April. Currently, DBM capture numbers are going down. Lower adults in the system could be paired with more caterpillars feeding on several host plants.

We have set up yellow sticky cards to track the overall population of winged aphids and thrips. These sticky cards are in the same locations as the pheromone traps. At this point, data for aphids and thrips is not broken down at the species level.

From the chart above, there was a flight of aphids during late March to early April. Some PCAs mentioned to me that foxglove aphid started to show up in their fields during that time period. It seems now that winged aphid numbers are going down. However, it does not mean that numbers of aphids in our crops are decreasing. We might be facing higher population of wingless aphids in our crops right now.

We need to keep an eye on population dynamics of thrips in the Valley. The ultimate goal is to be better prepared this season to manage those creatures and reduce the incidence of INSV virus. The chart below shows captures of thrips in our sticky cards.

It seems like thrips populations had a spike two weeks ago. Currently, thrips numbers are going down. I believe that keeping track of the fluctuation of thrips numbers in our Valley would help us detect large populations of this pest. There is a need to alert PCAs when the front of a 'thrips wave' would happen.

If you are interested in getting more information on this monitoring effort, please do not hesitate in contacting Alejandro Del-Pozo at 831-759-7359 or adelpozo@ucanr.edu.

Integrated pest management, commonly referred to as IPM, is a concept of managing pests that has been in use for several decades.  The definition and interpretation of IPM vary depending on the source, such as a university, institute, or a researcher, and its application varies even more widely depending on the practitioner.  Here are a few examples of its definitions and interpretations:

“IPM is an ecosystem-based strategy that focuses on long-term prevention of pests or their damage through a combination of techniques such as biological control, habitat manipulation, modification of cultural practices, and use of resistant varieties. Pesticides are used only after monitoring indicates they are needed according to established guidelines, and treatments are made with the goal of removing only the target organism. Pest control materials are selected and applied in a manner that minimizes risks to human health, beneficial and nontarget organisms, and the environment.” UC IPM

“Integrated Pest Management, or IPM, is an approach to solving pest problems by applying our knowledge about pests to prevent them from damaging crops, harming animals, infesting buildings or otherwise interfering with our livelihood or enjoyment of life. IPM means responding to pest problems with the most effective, least-risk option.” IPM Institute of North America

“A well-defined Integrated Pest Management (IPM) is a program that should be based on prevention, monitoring, and control which offers the opportunity to eliminate or drastically reduce the use of pesticides, and to minimize the toxicity of and exposure to any products which are used. IPM does this by utilizing a variety of methods and techniques, including cultural, biological and structural strategies to control a multitude of pest problems.” Beyond Pesticides

“IPM is rotating chemicals from different mode of action groups.” A grower

These definitions and interpretations represent a variety of objectives and strategies for managing pests.  IPM is not a principle that can/should be strictly and equally applied to every situation, but a philosophy that can guide the practitioner to use it as appropriate for the situation.  For example, varieties that are resistant to arthropod pests and diseases are available for some crops, but not for others.  Mating disruption with pheromones is widely practiced for certain lepidopteran and coleopteran pests, but not for several hemipteran pests.  Biological control is more readily employed for greenhouse pests, but not to the same extent under field conditions.  While chemical pesticides should be used as the last resort, in principle, sometimes they are the first line of defense to prevent damage to the transplants by certain pests or area-wide spread of certain endemic or invasive pests and diseases.

Crop production is an art, science, and business, and by adding environmental and social factors, IPM – an approach used in agriculture – can also be influenced by a number of factors.  Each grower has their own strategy for producing crops, minimizing losses, and making a profit in a manner that is acceptable to the society, safe for the consumers, and less disruptive to the environment.  In other words, “IPM is an approach to manage pests in an economically viable, socially acceptable, and environmentally safe manner”.  Keeping this simple, but loaded, definition in mind and considering recent advances in crop production and protection, communication technology, and globalization of agriculture and commerce, here is the new paradigm of IPM with its management, business, and sustainability aspects.

I. Management Aspect

There are four major components in the IPM model that address the various pest management options, the knowledge and resources the grower has in order to address the pest issue, planning and organization of information to take appropriate actions, and maintaining good communication to acquire and disseminate knowledge about pests and their management.

1. Pest Management:

The concept of pest control has changed to pest management over the years knowing that a balanced approach to managing pest populations to levels that do not cause economic losses is better than eliminating for environmental and economic reasons.  Although the term control is frequently used in literature and conversations, it generally refers to management.  A thorough knowledge of general IPM principles and various management options for all possible pest problems is important as some are preventive and others are curative.  It is also essential to understand inherent and potential interactions among these management options to achieve maximum control.  The following are common control options that can be employed at different stages of crop production to prevent, reduce, or treat pest infestations.  Each of them may provide only a certain level of control, but their additive effect can be significant in preventing yield losses.

a. Host plant resistance: It involves the use of pest resistant and tolerant cultivars developed through traditional breeding or genetic engineering.  These cultivars possess physical, morphological, or biochemical characters that reduce the plant's attractiveness or suitability for the pest to feed, develop, or reproduce successfully.  These cultivars resist or tolerate pest damage and thus reduce the yield losses.

b. Cultural control: Changing agronomic practices to avoid or reduce pest infestations and damage refers to cultural control.  Adjusting planting dates can help escape pest occurrence or avoid most vulnerable stages.  Modifying irrigation practices, fertilizer program, plant or row spacing, and other agronomic practices can create conditions that are less suitable for the pest.  Destroying crop residue and thorough cultivation will eliminate breeding sites and control soil-inhabiting stages of the pest.  Crop rotation with non-host or tolerant crops will break the pest cycles and reduce their buildup year after year.  Choosing clean seed and plant material will avoid the chances of introducing pests right from the beginning of the crop production.  Sanitation practices to remove infected/infested plant material, regular cleaning field equipment, avoiding accidental contamination of healthy fields through human activity are also important to prevent the pest spread.  Intercropping of non-host plants or those that deter pests or using trap crops to divert pests away from the main crop are some of the other cultural control strategies.

c. Biological control: Natural enemies such as spiders, predators, and parasitic wasps can be very effective in causing significant reductions in pest populations in certain circumstances.  Periodical releases of commercially available natural enemies or conserving natural enemy populations by providing refuges or avoiding practices that harm them are some of the common practices to control endemic pests.  To address invasive pest issues, classical biological control approach is typically employed where natural enemies from the native region of the invasive pest are imported, multiplied, and released in the new habitat of the pest.  The release of irradiated, sterile insects is another biological control technique that is successfully used against a number of pests.

d. Behavioral control: Behavior of the pest can be exploited for its control through baits, traps, and mating disruption techniques.  Baits containing poisonous material will attract and kill the pests when distributed in the field or placed in traps.  Pests are attracted to certain colors, lights, odors of attractants or pheromones.  Devices that use one or more of these can be used to attract, trap or kill pests.  Pheromone lures confuse adult insects and disrupt their mating potential, and thus reduce their offspring.

e. Physical or mechanical control: This approach refers to the use of a variety of physical or mechanical techniques for pest exclusion, trapping (in some cases similar to the behavioral control), removal, or destruction.  Pest exclusion with netting, handpicking or vacuuming to remove pests, mechanical tools for weed control, traps for rodent pests, modifying environmental conditions such as heat or humidity in greenhouses, steam sterilization or solarization, visual or physical bird deterrents such as reflective material or sonic devices are some examples for physical or mechanical control.

f. Microbial control: Using entomopathogenic bacteria, fungi, microsporidia, nematodes, and viruses, and fermentation byproducts of microbes against arthropod pests, fungi against plant parasitic nematodes, and bacterial and fungal antagonizers of plant pathogens generally come under microbial control. 

g. Chemical control: Chemical control typically refers to the use of synthetic chemical pesticides, but to be technically accurate, it should include synthetic chemicals as well as chemicals of microbial or botanical origin.  Although botanical extracts such as azadirachtin and pyrethrins, and microbe-derived toxic metabolites such as avermectin and spinosad are regarded as biologicals, they are still chemical molecules, similar to synthetic chemicals, and possess many of the human and environmental safety risks as chemical pesticides.  Chemical pesticides are categorized into different groups based on their mode of action and rotating chemicals from different groups is recommended to reduce the risk of resistance development.  Government regulations restrict the time and amount of certain chemical pesticides and help mitigate the associated risks.

The new RNAi (ribonucleic acid interference) technology where double-stranded RNA is applied to silence specific genes in the target insect is considered as a biopesticide application.  Certain biostimulants based on minerals, microbes, plant extracts, seaweed or algae impart induced systemic resistance to pests and diseases, but are applied as amendments without any claims for pest or disease control.  These new products or technologies can fall into one or more abovementioned categories. 

As required by the crop and pest situation, one or more of these control options can be used throughout the production period for effective pest management.  When used effectively, non-chemical control options delay, reduce, or eliminate the use of chemical pesticides.

2. Knowledge and Resources:

The knowledge of various control options, pest biology and damage potential, and suitability of available resources enables the grower to make a decision appropriate for their situation.

a. Pest: Identification of the pest, understanding its biology and seasonal population trends, damaging life stages and their habitats, nature of damage and its economic significance, vulnerability of each life for one or more control options, host preference and alternate hosts, and all the related information is critical for identifying an effective control strategy.

b. Available control options: Since not all control options can be used against every pest, the grower has to choose the ones that are ideal for the situation.  For example, systemic insecticides are more effective against pests that mine or bore into the plant tissue.  Pests that follow a particular seasonal pattern can be controlled by adjusting planting dates.  Commercially available natural enemies can be released against some, while mating disruption works well against others.  Entomopathogenic nematodes can be used against certain soil pests, bacteria and viruses against pests with chewing mouthparts such as lepidoptera and coleopteran, and fungi against sucking pests.

c. Tools and technology: A particular pest can be controlled by certain options, but they may not all be available in a particular place, for a particular crop, or within the available financial means.  For example, the release of natural enemies may be possible in high-value speciality crops, but not in large acreage field crops.  A particular pesticide might be registered against a pest on some crops, but not on all.  Use of netting or tractor-mounted vacuums can be effective, but very expensive limiting their availability to those who can afford.

This is a critical component where diagnostic and preventive or curative decisions are made based on available and affordable control options.

3. Planning and Organization:

This component deals with the management aspect of the of the new IPM model for data collection, organization, and actual actions against pest infestations.

a. Pest monitoring: Regularly monitoring the fields for pest infestation and spread is a basic step in crop protection.  Early detection in many cases can help address the pest situation by low-cost spot treatment or removal of pests or infected/infested plant material.  When pest infestations continue to grow, regular monitoring is necessary to assess the damage and determine the time to initiate farm-wide control.  Monitoring is also important to avoid calendar-based pesticide applications especially at lower pest populations that do not warrant treatments.

b. Managing information: A good recordkeeping about pests, their damage, effective treatments, seasonal fluctuations, interactions with environmental factors, irrigation practices, plant nutrition, and all related information from year to year will build the institutional knowledge and prepares the grower to take preventive or curative actions.

c. Corrective actions: Taking timely action is probably the most important aspect of IPM.  Even with all the knowledge about the pest and availability of resources for its effective management, losses can be prevented only when corrective actions are taken at the right time.  Good farm management will allow the grower to take timely actions.  These actions are not only necessary to prevent damage on a particular farm, but also to prevent the spread to neighboring farms.  When pest management is neglected, it leads to area-wide problems with larger regulatory, social, and economic implications.

4. Communication:

Good communication to transfer the individual or collective knowledge for the benefit of everyone is the last component of the new IPM model.  Modern and traditional communication tools can be used for outreach as university and private researchers develop information about endemic and invasive pests, emerging threats, and new control strategies.

a. Staying informed: Growers and pest control professionals should stay informed about existing and emerging pests and their management options.  Science-based information can be obtained by attending extension meetings, webinars, or workshops, reading newsletter, trade, extension, or scientific journal articles, and keeping in touch with researchers and other professionals through various communication channels.  Well-informed growers can be well prepared to address pest issues.

b. Communication within the group: Educating farm crew through periodical training or communication will help with all aspects of pest management, proper pesticide handling, ensuring worker safety, and preventing environmental contamination.  Knowledgeable field crew will be beneficial for effective implementation of pest management strategies.

c. Communication among growers: Although certain crop production and protection strategies are considered proprietary information, pests do not have boundaries and can spread to multiple fields when they are not effectively managed throughout the region.  Sharing knowledge and resources with each other will improve pest control efficacy and benefit the entire grower community.

In addition to these four components with an IPM model, factors that influence profitable, safe, and affordable food production at a larger scale and their implications for global food security should also be included.  There are two layers surrounding these four components addressing the business and sustainable aspects of food production. 

II. Business Aspect:

Consumers want nutritious, healthy, and tasty produce that is free of pest damage at affordable prices.  Growers try to meet this demand by producing food that meets all the consumer needs, while maintaining environmental and human safety and still being able to make a profit.  Sellers evaluate the market demand and strategize their sales to satisfy consumers while making their own profit to stay in the business.  In an ideal system, consumer, producer, and seller would maintain a harmonious balance of food production and sale.  In such a system, food is safe and affordable to everyone, there will be food security all over the world, and both growers and sellers make a good profit with no or minimal risk to the environment in the process of food production.  However, this balance is frequently disrupted due to i) consumers' misunderstanding of various food production systems, their demand for perfectly shaped fruits and vegetables at affordable prices or their willingness to pay a premium price for food items that are perceived to be safe, ii) growers trying to find economical ways of producing high quality food while facing with continuous pest problems and other challenges, and iii) sellers trying to market organic food at a higher price as a safer alternative to conventionally produced food.  If growers implement good IPM strategies to produce safe food and consumers are aware of this practice and gain confidence in food produced in an IPM system, then sellers would be able to market what informed-consumers demand.

III. Sustainability Aspect:

As mentioned earlier, IPM is an approach to ensure economic viability at both consumer and producer level (seller is always expected to make a profit), environmental safety through a balanced use of all available pest control options, and social acceptability as food is safe and affordable. 

While organic food production is generally perceived as safe and sustainable, the following examples can explain why it is not necessarily true.  Organic food production is not pesticide-free and some of the pesticides used in an organic system are as harmful to humans and non-target organisms as some chemical pesticides.  Certain organically accepted pesticides have toxins or natural chemical molecules that are very similar to those in synthetic pesticides.  In fact, some synthetic pesticides are manufactured imitating the pesticidal molecules of natural origin.  Mechanical pest control practices such as vacuuming or tilling utilize fossil fuels and indirectly have a negative impact on the environment.  For example, diesel-powered tractors are operated for vacuuming western tarnished bug in strawberry 2-3 times or more each week while a pesticide application typically requires the use of tractor once every 7-14 days.  To control certain pests, multiple applications of organic pesticides might be necessary with associated costs and risks, while similar pest populations could be controlled by fewer chemical pesticide applications.  It is very difficult to manage certain plant diseases and arthropod pests through non-chemical means and inadequate control not only leads to crop losses, but can result in their spread to larger areas making their control even more difficult.  Many growers prefer a good IPM-based production to an organic production for the ease of operation and profitability.  However, they continue to produce organic food to stay in business.

While middle and upper-class consumers may be willing to pay higher prices for organically produced food,  many of the low-income groups in developed and underdeveloped countries cannot afford such food. Organic food production can lead to social inequality and a false sense of wellbeing for those can afford.  Food security for the growing world population is necessary through optimizing input costs, minimizing wastage, grower adoption of safe and sustainable practices, and consumer confidence in food produced through such practices.  IPM addresses all the economic, environmental, and social aspects and provides safe and affordable food to the consumers and profits to producers and sellers, while maintaining environmental health.

Leaf spots are a common issue that affects spinach and reduces its salability. Spots on spinach leaves are caused by both biotic and abiotic influences. Insects, such as leafminers, frequently cause spots on spinach leaves. Female leafminers stipple spinach leaves by puncturing the leaf surface with their ovipositors and then feeding on plant sap that exudes from the holes. The stippled areas often occur in clusters and have a characteristic look due to the broken epidermal cells in the center of the stipple (photo below).

Diseases that cause spots on lettuce include the following: Cladosporium causes round, tan lesions that have dark green spores and mycelia in the center of the spots (http://ipm.ucanr.edu/PMG/r732100311.html); Anthracnose lesions start as dark green water-soaked lesions that later turn tan with black fruiting bodies in the center (can be observed with a good hand lens, http://ipm.ucanr.edu/PMG/r732100211.html); Stemphylium causes circular lesions, but no fungal fruiting bodies or mycelia occur in the lesions making it difficult to distinguish this disease from abiotic causes (http://ipm.ucanr.edu/PMG/r732100411.html).

Stippling on a spinach leaf from leafminer feeding

Abiotic leaf spots on spinach are caused by a variety of factors. The most common cause is from burn caused by herbicides, other pesticides or fertilizers; in addition, water and other stresses can cause spotting or lesions on spinach. Spinach leaves are quite sensitive to chemicals and will readily respond to them by developing chlorotic or tan colored necrotic areas. The size and distribution of the lesions can often provide clues as to the cause of the issue. For instance, spots caused by herbicides or other chemicals (Photos No. 1) often have a characteristic pattern which can reveal if the burn was caused by spray drift, herbicide on dust or even a direct spray. Spray drift or lift off of oxyfluorfen often causes small diffuse spotting on the leaves. The location of the affected plants in the field can indicate the direction that the chemical came from. Also, examining the pattern of the lesions on the plant can give an indication when the drift incident may have occurred, depending on which age of leaves are affected; for instance, younger leaves may be unaffected because they were protected down in the crown of the plant when the incident occurred. Also, if only the outer edge of leaves are affected, this may indicate that the base of the leaves were protected by shingling of other leaves; this symptom often helps to confirm that you are dealing with a drift issue vs some other cause. In many of these types of situations, having some background information about recent spray applications in the vicinity of the field helps piece together how and when the incident occurred. Spotting on the weeds also provides confirmation of the cause of the incident. Distortion of the leaves (Photos No. 2) occurs when the necrosis occurs early in the development cycle of the leaf; in this situation, the expanding young leaf continues to develop around the dead lesion and results in distorted growth. Chemical issues can also cause a sub-lethal response in spinach leaves which results in chlorotic lesions (Photos No. 3).

Other chemicals that commonly cause burn on spinach include salts. Salts in water can cause spotting and marginal burns on spinach (Photos No. 4), but it is rare for water to be salty enough to cause this issue. However, water-run injections of fertilizer are salty enough if the injected fertilizer is not adequately rinsed from the irrigation lines after the injection. Fertilizer burn from water runs typically look different than spray burn because the lesions often occur along the edge of the leaves and are typically larger and blotchier (Photos No. 5). Sometimes growers are surprised that their spinach was burned from fertilizer because they routinely apply fertilizer through the sprinkler with no issues; however, if the clean-out phase of the injection was not long enough, then fertilizer burn can readily occur. Topdress applications with dry fertilizer at the 1^st – 2^nd true leaf stage can also cause burn on spinach leaves if the fertilizer prills stick to moisture on the leaves (Photos No. 6).

Most of the spotting that we see on spinach is from the above-mentioned causes. However, other issues that can cause defects on the leaves include water stress (Photos No. 7) which is often tied to hot spells. Water stress characteristically will occur in large blotches in the interveinal region of the leaf. It mostly occurs on older to mid-aged leaves and on various locations on the leaf. Hail damage (Photo No. 8) occasionally occurs in the spring and causes light green spots due to damaged epidermal cells that lets the green from tissue lower in the leaf to show through. Tipburn of spinach occurs in the spring when air temperatures increase, but soil temperatures are still cool. Under such conditions a localized deficiency of calcium develops out on the tip of the young leaf; as the leaf continues to expand, the necrotic tissue on the tip inhibits expansion resulting in a “hooded” shape to the leaf (Photos No. 9). Occasionally, chimeras are seen in spinach fields which are the result of a mutation that occurs in the meristem of the leaf; the resulting leaves have a dramatic calico yellow and green appearance (Photos No. 9).

1. Herbicide or other Chemical Burn

Chateau moved on dust moved large distances across this field

Fine, evenly-distributed pattern of herbicide spray drift; sometimes only the tips of some leaves are affected is they are protected by an upper leaf.

Fine, evenly-distributed pattern of herbicide drift

  

Heavy, blotchy distribution of herbicide

The weeds often show spotting from herbicide burn as well

2. Necrosis and Distortion

Spotting/necrosis that occurs on young, expanding leaves causes distortion as the leaf continues to grow around the damaged tissue

3. Yellow Lesions

Possible Spin-Aid damage on freezer spinach

  

Fine pattern of yellow lesions (sublethal chemical burn)

4. Salts in Water

High levels of salts in irrigation water can burn spinach; usually the damage is spots on the leaf surface or more commonly, on the margins of the leaves

5. Water-run Fertilizer Burn

Burn from water-run fertilizer applications is similar to salt burn shown above. The burn will most often occur along the margins of the leaves and occur as large blotchy lesions

6. Dry, Topdress Fertilizer Burn

Dry fertilizer burn from topdress applications can burn the leaves in discrete spots on the edges or other parts of the leaf

7. Water Stress

Water stress symptoms often occur as interveinal and blotchy necrotic areas on the leaf

8. Hail Damage

Hail damage causes damage to the epidermis of the leaf giving a light green appearance to the lesion

9. Other Leaf Issues

Tipburn causes a hooded shape to the leaf

  

Chimera

Background

Testing the soil for nitrate is critical for managing fertilizer in crop production. Soil nitrate levels are continually in flux due to inputs of nitrate from fertilizer, mineralization of soil organic matter and crop residues, and irrigation water, as well as from losses of soil nitrate from leaching, crop uptake and denitrification.  As a result, we advise to measure soil nitrate as close to a fertilization event as possible to make your decision based on current soil nitrate levels. Soil samples can also be sent to a laboratory for nitrate analysis, but there can be a lag in getting the results back which can reduce the usefulness of the analysis for making fertilizer decisions.

The soil nitrate quick test has the advantage over laboratory tests of analyzing soil nitrate in a timely fashion so that accurate fertilizer application decisions can be made. The soil nitrate quick test is often used just before a fertilizer application. If soil nitrate values are high enough, it is possible to reduce the fertilizer rate or even skip the fertilizer application without jeopardizing crop yield because the existing nitrate in the soil supplies the plant with nitrogen in the same way as applied fertilizer. 

Procedure

Soil cores are taken to a 12-inch depth for lettuce and spinach; however, for deeper rooted crops such as broccoli and cauliflower, soil cores of the second foot of soil during the last half of the crop cycle provide additional information on residual soil nitrate available for crop growth. Scrape away the soil from the top 2 inches of soil as it may be high in nitrate (due to upward movement of salts), but too dry for the plants to access. We have found that on some soil types (e.g. clays, silty clays) it is important to angle the soil probe in the direction of the fertilizer bead or drip tape (in fertigated situations) (See Figures 1 & 2). The reason for this is that in these soils, the fertilizer sometimes does not move far with the irrigation water and by angling the probe, you collect a more representative sample.  As a matter of habit, we angle the probe on all soil types to keep our sampling method uniform.

Sample the field in a pattern that goes from one end of the field to the other, both sides of the field and through the middle – generally an “X” or “N” shaped pattern is fine.  For a representative sample, it is important to collect enough samples, generally, 15 to 20 soil cores.

After collection, the sample needs to be thoroughly homogenized. Sandy soils are easily homogenized, but sticky clays or even wet loams are too gummy to mix. In these situations, we do the “pinch” method by laying out the soil cores and pinching off small, uniform amounts of soil from up and down each core. We then mix the pinches and use them for placing in the calcium chloride solution (see below). The strips are read with colorimetric test strips (see photo). The cheapest are the MQuant nitrate test strips described below. They can also be read with the Reflectoquant reader which provides a more accurate reading of the color development using Reflectoquant test strips.  

Figures 1 & 2. Insert the probe in the seedline, but angle it to go beneath the bead of fertilizer or beneath the drip tape.

Procedure for conducting the nitrate quick test:

Equipment Needed

• MQuant 1.10020.0001 nitrate and nitrite test strips (0 to 500 ppm nitrate). They are available from MilliporeSigma or Amazon and contain 100 strips.  They should be stored in a refrigerator when not being used for field testing.  Also because color development may change as the strips age, it is advisable to store solutions of known nitrate concentration in a refrigerator to test if the strips are still accurate.
• 50 ml polyethylene centrifuge tubes (Figure 3) and a rack to hold sample tubes. These can be ordered from scientific supply companies, but they want to sell large batches that cost more. Amazon will sell smaller batches that are cheaper.
• Calcium chloride dihydrate. Can be ordered from scientific supply companies, but aquarium or food grade (e.g. canning supply companies or bulkfoods.com) calcium chloride is also fine for conducting the test.
• 1 gallon of distilled water
• Add 5.6 grams of calcium chloride to 1 gallon of distilled water to make up the 0.01 M calcium chloride solution

 Note: Nitrate test strips should be stored in a refrigerator when not being used for field testing.  Also because color development may change as the strips age, it is advisable to store solutions of known nitrate concentration in a refrigerator to test if the strips are still accurate.  Using water samples from several wells with different concentrations of nitrate could be used to test if the strips continue to provide consistent readings. 

Procedure

1. Collect a composite soil sample as described above.
2. Fill a volumetrically marked tube or cylinder to the 30 ml level with 0.01 M Calcium Chloride (CaC1₂) solution.
3. Add soil to the tube until the liquid level rises to 40 ml; cap tightly and shake vigorously until soil is thoroughly dispersed.  Let sit until soil particles settle out.
4. When solution is reasonable clear, dip the nitrate test strip into the solution, shake off excess solution, and wait 60 seconds.  Compare color with the color chart provided (Figure 4)
5. To minimize variability inherent in soil sampling, run duplicate samples for each field soil evaluated.

Figures 3.  An empty polyethylene centrifuge tube.   Figure 4.  Dip the test strip in the clear supernatant and allow it to develop color for 1 minute

Figure 5. Use the scale indicated by red arrow for calculating soil nitrate concentration for mQuant test strips.

Interpretation

The MQuant test strips are calibrated both in parts per million ppm NO₃ and ppm NO₃-N.   Reading the ppm NO₃ value (Figure 5), use the table and equation below to convert the reading to ppm NO₃-N in dry soil:

Strip reading (ppm NO3) ÷ correction factor = ppm NO₃-N in dry soil

 For instance, a reading from the test strips of 25 ppm NO₃ from a moist loam would have 12.5 ppm NO₃-N in the soil (25 ÷ 2 = 12.5). The ppm NO₃-N values can be converted to pounds of nitrogen per acre in the top foot of soil by multiplying by 3.7, and in this example, that would equal 46 pounds of nitrogen per acre.

 In general, soils with less than 10 ppm NO₃-N are considered low for fast growing vegetable crops and soils with levels above 20 ppm NO₃-N may have enough available N to supply crop needs for a limited period. Intermediate concentrations between 12 and 15 ppm NO₃-N may warrant a half rate of fertilizer. However, it is important to get familiar with the nitrate quick test by doing small trials on your farm. As you gain more confidence in using the test to adjust fertilizer applications, you can do larger trials. Keep in mind that nitrate is very mobile, and in light textured soils, heavy irrigation/rainfall events can reduce the amount of available nitrogen in the soil.  That is why it is always good to be cautious in reducing fertilizer applications based on the soil nitrate test.  Feel free to contact either of us if you have any questions.

Additional information resources on soil nitrate testing and nitrate in irrigation water:

 Using the Pre-Sidedressing Soil Nitrate ‘Quick Test' to Guide N Fertilizer Management

https://afghanag.ucdavis.edu/irrigation-natural-resource/files/soil-nitrate-quick-test.pdf

Accuracy of Test Strips for Assessing Nitrate Concentration in Soil and Water

https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=13140

Sampling for Soil Nitrate Determination

https://apps1.cdfa.ca.gov/fertilizerresearch/docs/Soil_Sampling_Nitrate.pdf

RQflex reader can improve the accuracy of nitrate test strips

https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=25314

Presidedress nitrate quick test reduces nitrate leaching hazard in lettuce

http://www.ucanr.org/sites/nm/files/76758.pdf

Nitrate to nitrogen conversion and estimating N contribution from irrigation waters containing nitrate

https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=7744

Estimating N contribution from irrigation water containing nitrate

https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=9361