Concurrent Session 2C
Root-Knot Nematode Species Identification Using Mitochondrial DNA (2011-2013)
Valerie Williamson, Professor, Dept. of Nematology, UC Davis
The goal of the project was to develop a straightforward, rapid DNA-based procedure to identify root knot nematode (RKN) species to tailor the control strategy to the species. A DNA-based assay that differentiates the key species was developed and optimized for routine identification. This assay can identify the species of nematode samples preserved in ethanol, allowing for transport from the field and storage and has the potential to discriminate and identify novel and invasive species. The information obtained is being used to advise growers on sustainable practices and to develop new tomato varieties resistant to nematode species for which resistance is currently not available. Held a training session and distributed protocols to seed companies for use in resistance breeding programs and for assessing resistance-breaking nematode strains from growers. Interacting with California Department of Food and Agriculture personnel regarding incorporation of this diagnostic assay.