Fundamental and Advanced Applied Nematology Workshop
Co-organizers: John Chitambar, Ke Dong, and Sergei Subbotin, California Department of Food and Agriculture, Nematology Laboratory, Plant Pest Diagnostics Branch, Sacramento, California
Sunday November 6, 2011, 8:30 am - 4:30 pm
Fundamental Applied Nematology Workshop
$100 per person includes lunch
The workshop will include lecture, demonstration, hands-on applications and discussions. In the morning session participants will be introduced to a general biology of plant parasitic nematodes, plant disease signs and symptoms, general sampling procedures, nematode extraction techniques, and an introduction to nematode morphology. Hands-on applications will cover preparation of temporary whole mounts of nematodes, perennial patterns of root-knot female nematodes, and vulval cones of cyst nematodes for microscopic examination. Also, included will be microscope identification of certain regulatory and economic important species of plant parasitic nematodes. Participants will receive a printed manual and a box of essential diagnostic tools to take with them.
In the afternoon session participants will be introduced to basic molecular diagnosis of plant parasitic nematodes. This will include lecture and demonstration of the PCR technique for DNA identification. The remainder duration will be allotted to hands-on preparations of whole mounts of nematodes, perennial patterns, vulval cones and microscope identification of certain regulatory and economic important species of plant parasitic nematodes, as introduced in the morning session.