Current Studies: Bacterial Isolates
Microbial culturing is a method of multiplying an organism on a specific media under controlled laboratory conditions. The isolation and maintenance of pure cultures of an organism is essential to research. Importantly, the maintenance of pure cultures also provides a tool for determining the cause of disease symptoms.
In preparation for future studies on P. savastanoi, we will initiate work to collect, archive, and store isolates of the P. savastanoi bacterium across the olive-growing regions in California.
This effort will provide isolates of this pathogen that could then be used in trials assessing cultivar susceptibility, and included in phylogenetic and other studies.
Fig. 1. Excision of gall tissue allows for pathogen isolation and identification.
Fig. 2. Colonies of P. savastanoi floresce under ultra-violet light.
|Photos by Elizabeth Fichtner, UCCE Tulare Co., CA|