Current Studies: Pathogen Survival
An experimental study on in planta pathogen survival was initiated in April 2011. Leaf scars were mechanically exposed on flagged branches of 7 mature Manzanillo trees. Leaf scars were then artificially inoculated with a 106 cfu/ml bacterial suspension of P. savastanoi. The bacterial inoculum was comprised of a cocktail of 9 P. savastanoi isolates collected from gall tissues at UC Davis in October 2010.
Leaves were mechanically abscised from 10 nodes and leaf scars were then artificially inoculated with P. savastanoi. Photo by Elizabeth Fichtner, UCCE Farm Advisor, Tulare Co., CA
Resulting galls will be sampled over successive years to generate a pathogen survival curve within gall tissues. Data collected will be both qualitative and quantitative, illustrating presence/absence of the pathogen as well as pathogen population dynamics in symptomatic tissues. Additionally, production of secondary galls (ie. movement of inoculum within the plant) will be assessed beyond the point of original inoculation.