- Author: Kathy Keatley Garvey
He and other members of the research team are exploring the intact organs and tissues of tsetse flies using a powerful 3D X-ray imaging technique. The study, “Unraveling Intersexual Interactions in Tsetse”), is funded by the National Institute of Allergy and Infectious Diseases (NAIAD) of the National Institutes of Health.
“We started this project in 2019 and the work is ongoing,” said Attardo, an assistant professor in the UC Davis Department of Entomology and Nematology and chair of the Designated Emphasis in the Biology of Vector Borne Diseases. “We actually have scans of flies through the entire reproductive cycle, however, the segmentation is ongoing. We are working on developing ways to train artificial intelligence based software to assist us with the tissue segmentations.”
The tsetse fly transmits the parasite that causes the deadly human and animal trypanosomiasis, better known as African sleeping sickness, says Attardo, who is featured in a recently posted article, "A Detailed Look Inside Tsetse Flies," on the Berkeley Lab website. (See YouTube)
“The imaging technique provided new insights into how the flies' specialized biology governs mating and reproductive processes, including female flies' unique lactation and their delivery of a single fully developed larvae per birthing cycle – whereas most other insect species lay eggs,” according to the article. “The ALS (National Laboratory Advanced Light Source) produces X-rays and other forms of light for a broad range of simultaneous scientific experiments.”
Attardo, who specializes in medical entomology, reproductive physiology, molecular biology and genetics, says that tsetse flies resemble house flies, but are distinguished from other Diptera by their unique adaptations, including lactation and the birthing of live young. They carry only one offspring in their uterus at one time.
The parasite invades the central nervous system and disrupts the sleep cycle, says Attardo. “If not treated, the disease can result in progressive mental deterioration, coma, systemic organ failure and death.” An estimated 65 million people in 36 countries in sub-Saharan Africa are at risk for the deadly disease, according to the World Health Organization.
Attardo led a study, published in September 2020 in the journal Insects, detailing the ALS imaging work. The article, “Interpreting Morphological Adaptations Associated with Viviparity in the Tsetse Fly Glossina morsitans (Westwood) by Three-Dimensional Analysis,” received widespread attention. ALS experiments allow the researchers to create a detailed 3D visualizatiaon of the reproductive tissues without dissection and staining processes that introduce damage to the delicate samples.
“We want to understand what changes are happening during this process, how the process is being mediated, and if it can be manipulated to artificially repress females in the wild from mating,” Attardo told the Berkeley Lab News Center.
The Berkeley Lab is a multiprogram science lab in the national laboratory system supported by the U.S. Department of Energy through its Office of Science.
In his UC Davis lab, Attardo researches one of 35 tsetse fly species, Glossina morsitans morsitans, which prefers feeding on cattle to humans. Its strong mouthparts can easily puncture the tough cattle hide. In his lab, he feeds them warm cow blood.
Attardo holds a doctorate in genetics from Michigan State University, where he researched the molecular biology of mosquito reproduction in the lab of Alexander. Prior to joining the UC Davis faculty in 2017, Attardo worked for 13 years in the Department of Epidemiology of Microbial Diseases at the Yale School of Public Health, first as a postdoctoral associate and then as a research scientist studying the reproductive biology of tsetse flies.
- Author: Sean Nealon
The team, led by Samantha Ying, an assistant professor of environmental sciences at UC Riverside, received the grant from the University of California Office of the President.
The funding will allow for the establishment of the University of California Consortium for Drought and Carbon Management (UC DroCaM), which will design management strategies based on understanding soil carbon, the soil microbiome and their impact on water dynamics in soil.
The researchers will conduct field and lab research on microbiological, biophysical, and geochemical mechanisms controlling soil formation and stability under different row crops (tomatoes, alfalfa, wheat), farming practices (carbon inputs and rotations) and irrigation methods (furrow and flood, microirrigation).
Field research will initially be conducted at three UC Research and Extension Centers (Kearney, West Side and Desert) the Russell Ranch Sustainable Agriculture Facility near UC Davis.
Recommendations will then be made for broader monitoring and field experiments throughout the state based on input gained from local growers and citizens at workshops at the agricultural research stations. Ultimately, the hope is to expand and involve all nine research and extension centers from the Oregon border to the Mexican border.
“Having agricultural research stations throughout the state is a huge part of this project,” Ying said. “It is going to help us create one of the best research centers in the country focused on soil and drought.”
There is also a public engagement component. Citizens will be recruited to participate in workshops to learn how to monitor and sample their local soils. Information will then be imputed into an online soils database that will help create a map of the biodiversity of agricultural soils in California.
Ying's collaborators are: Kate Scow and Sanjai Parihk (UC Davis); Eoin Brodie and Margaret Torn (UC Berkeley); Asmeret Berhe and Teamrat Ghezzehei (UC Merced); and Peter Nico and William Riley (Lawrence Berkeley National Laboratory).
The grant is one of four awards totaling more than $4.8 million from University of California President Janet Napolitano's President's Research Catalyst Awards.