Butterflies, beer and a bar...Who wants to drink to science?

If you've ever wanted to converse with butterfly guru Art Shapiro, UC Davis distinguished professor of evolution and ecology, about "butterflies and the  apocalypse" and sip a beer (or another beverage) at the bar at the same time, here's your chance.

The Davis Science Café has booked "A Conversation with Arthur Shapiro: Butterflies as Heralds of the Apocalypse" at 5:30 p.m., Wednesday, April 10 in the G Street Wunderbar, located at 228 G St., Davis. The event, hosted by professor Jared Shaw, professor and interim department chair of the UC Davis Department of Chemistry, is free and open to the public (but the refreshments  are not).

Shapiro has monitored butterfly population trends on a transect across central California since 1972. The 10 sites stretch from the Sacramento River Delta through the Sacramento Valley and Sierra Nevada mountains to the high desert of the Western Great Basin. The largest and oldest database in North America, it was recently cited by British conservation biologist Chris Thomas in a worldwide study of insect biomass.

A favorite of the news media, Shapiro has recently been featured in Newsweek, Washington Post, New York Times and the Los Angeles Times. Bay Nature magazine writer Eric Simons recently interviewed him for his piece on "What Do You Do About the Insect Apocalypse?"

What's going on with the butterflies?

The overwintering western population of the monarch butterfly on the central California coast declined 86 percent last winter,  the Xerces Society for Invertebrate Conservation indicates on its website. "Working at a conservation nonprofit means that we often come across bad news, but the results from this winter's Western Monarch Thanksgiving Count left us shocked: an all-time record low of 28,429 monarchs at 213 sites. This number is an 86% drop from the previous count done at Thanksgiving 2017, when 192,668 monarchs were counted at 263 sites (comparing only the sites monitored in both years)—and a dizzying 99.4% decline from the numbers present in the 1980s (Schultz et al. 2017). In short, only one of every 160 monarchs present in the 1980s exists today."

At the 2018 Butterfly Summit at Annie's Annuals and Perennials in Richmond, Shapiro told the crowd that "The vast majority of the butterflies we monitor are emerging earlier in the year now than they were in the 1970s."

His research shows that not only are butterflies coming out earlier, but "we also find trends in population and species richness."

Shapiro, a member of the UC Davis faculty since 1971 and author of the book, Field Guide to Butterflies of the San Francisco Bay Area and Sacramento Valley Regions, said that "in a nutshell, at low elevations, butterfly faunas have been declining slowly until 1999. In 1999, 17 species had an abrupt fall in abundance, spontaneously. On its face, this was a non-random event. The decline was then rapid from 1999 to the onset of the recent drought and then things went up again."

Science Café, initially supported by the National Science Foundation, is currently supported by the Department of Chemistry and Division of Mathematical and Physical Sciences and promoted by Capital Science Communicators.

Science Café sessions are hosted the second Wednesday of each month.  Topics so far this year? Plant ecologist Mark Schwartz of the UC Davis Department Environmental Sciences and Policy held forth at the March session on "Does California Have a Wildfire Problem? Can It Be Fixed?"  The February session featured Professor Roland Faller of the UC Davis Department of Chemical Engineering on "Using Computers to Understand Materials: From Proteins to Semiconductors" while Professor Denis Marcellin-Little of the UC Davis School of Veterinary Medicine's Surgical and Radiological Sciences discussed "Don't Try That at Home; High-End 3D Printing in Orthopedic Surgery" at the January session.

So it's butterflies and the apocalypse on April 10. Ask your questions, sip an adult beverage (or another beverage) and enjoy the evening!

 Bruce Hammock, distinguished professor at the University of California, Davis, who holds a joint appointment with the Department of Entomology and Nematology and the UC Davis Comprehensive Cancer Center, is fond of saying that "Science is full of surprises."

A member of the UC Davis faculty since 1980, Hammock received his doctorate in entomology and toxicology from UC Berkeley, where he studied insect science. He now devotes his research to human health.

What many people do not know, however, is that he began his career studying how caterpillars turn into butterflies.

That morphed into human health research.

“The work led to the discovery that many regulatory molecules are controlled as much by degradation and biosynthesis,” Hammock related. “The epoxy fatty acids control blood pressure, fibrosis, immunity, tissue growth, pain and inflammation to name a few processes.”

 Fast forward to today.

An enzyme inhibitor developed in the Hammock lab and tested in mice by a team of international researchers shows promise that it could lead to a drug to prevent or reduce the disabilities associated with the neurodevelopmental disorders of autism and schizophrenia.

What the Inhibitor Did
"We discovered that soluble epoxide hydrolase (sEH) plays a key role in inflammation associated with neurodevelopmental disorders. Inhibiting that enzyme stops the inflammation and the development of autism-like and schizophrenia-like symptoms in animal models,” said collaborator Kenji Hashimoto, a professor with the Chiba University Center for Forensic Mental Health, Japan. The scientists found higher levels of sEH in a key region of the brain—the prefrontal cortex of juvenile offspring-- after maternal immune activation (MIA).

“Mothers who have MIA, which results from severe stress in that region of the brain, have an increased occurrence of neurodevelopment disorders in their offspring,” Hashimoto explained. “In our study, the sEH enzyme increased dramatically in a key brain region of mice pups from mothers with MIA.”

The news embargo lifted today (March 18) on their research, to appear in the Proceedings of the National Academy of  Sciences (PNAS). (Link will be here: https://www.pnas.org/cgi/doi/10.1073/pnas.1819234116.) It's the work of 14 researchers from Chiba University Center for Forensic Mental Health; the Laboratory for Molecular Psychiatry, RIKEN Center for Brain Science, in Wako, Saitama, Japan; and the Hammock laboratory.

Reversed Cognitive and Sciatl Interaction Deficiencies
By inhibiting sEH, the researchers reversed cognitive and social interaction deficiencies in the mice pups. They hypothesize that this is due to increasing natural chemicals, which prevent brain inflammation. In people, this could reduce the disabilities associated with autism, such as anxiety, gastrointestinal disturbances and epilepsy.

Earlier studies have indicated a genetic disposition to the disorders. The team also studied postmortem brain samples from autism patients that confirmed the alterations.

“In the case of both autism and schizophrenia, the epidemiology suggests that both genetics and environment are contributing factors,” said neuroscientist and associate professor Amy Ramsey of the Department of Pharmacology and Toxicology, University of Toronto, who was not involved in the study. “In both cases, maternal infection is a risk factor that might tip the scales for a fetus with a genetic vulnerability. This study is important because it shows that their drug can effectively prevent some of the negative outcomes that occur with prenatal infections. While there are many studies that must be done to ensure its safe use in pregnant women, it could mitigate the neurological impacts of infection during pregnancy.”

May Lead to Important Therapeutic Intervention
Neuroscientist Lawrence David, professor and chair of the School of Public Health, University of Albany, N.Y., who was not involved in the research, said that the study might lead to “an important therapeutic intervention for neurodevelopment disorders.”

“There is increasing evidence that maternal immune activation activities (MIA) during fetal development can lead to aberrant neurobehaviors, including autistic-like activities,” said Lawrence, who studies neuroimmunology and immunotoxicology. The study “suggests that enzymatic control of fatty acid metabolism is implicated in neuroinflammation associated with schizophrenia and autism spectrum disorders. The expression of Ephx2 giving rise to soluble epoxide hydrolase (sEH) influences production of fatty acid metabolites, which elevate inflammation in the experimental model of mice after MIA; the sEH inhibitor TPPU (N-[1-(1-oxopropyl)-4-piperidinyl]-N'-[4-(trifluoromethoxy)phenyl)-urea) was postnatally used to improved behaviors. Analysis of cadaver brains from individuals with ASD also expressed increased sEH. Fatty acid metabolites have been known to affect fetal development, especially that of the brain; therefore, TPPU might be an important therapeutic intervention for neurodevelopmental disorders.”

Molecular bioscientist Isaac Pessah of the UC Davis School of Veterinary Medicine, distinguished professor and associate dean of research and graduate education in the Department of Molecular Biosciences, described the findings as “significant” and called for more detailed and expanded studies.

“There is mounting evidence that inappropriate maternal immune responses during pregnancy to infection contributes elevated risk to autism spectrum disorder, at least in a fraction of cases,” Pessah said. “The most significant findings reported here is that a commonly used mouse model of immune-triggered behavioral deficits mimicking some of the core symptoms in autistic children can be suppressed by inhibiting a novel biochemical target, soluble epoxide hydrolase; a target not previously explored as a target for therapeutic intervention to treat ASDs. These findings provide a rational basis for more detailed and expanded studies in mice carrying mutations implicated in ASDs to determine whether the therapeutic benefits of soluble epoxide hydrolase inhibitor(s) observed in this study are more generalizable.”

Autism: 1 of 68 Children
The Center for Disease Control and Prevention (CDC) estimates that 1 in 68 children in the United States have autism, commonly diagnosed around age 3. It is four times more common in boys than girls. CDC defines autism spectrum disorder as a “developmental disability that can cause significant social, communication and behavioral challenges.” The disorder impairs the ability to communicate and interact.

Schizophrenia: 1.2 Percent of Population
Approximately 3.5 million people or 1.2 percent of the population in the United States are diagnosed with schizophrenia, one of the leading causes of disability, according to the Schizophrenia and Related Disorders Alliance of America (SARDAA). Scores more go unreported. Approximately three-quarters of persons with schizophrenia develop the illness between 16 and 25 years of age. Statistics also show that between one-third and one half of all homeless adults have schizophrenia, and 50 percent of people diagnosed have received no treatment. Among the symptoms: delusions, hallucinations, disorganized speech, disorganized or catatonic behavior, and obsessive-compulsive disorders, such as hoarding, according to SARDAA.

In their research paper, titled “Key Role of Soluble Epoxide Hydrolase in the Neurodevelopmental Disorders of Offspring After Maternal Immune Activation,” the scientists described sEH as “a promising prophylactic or therapeutic target for neurodevelopmental disorders in offspring after MIA.”

First author Min Ma and second Qian Ren of the Hashimoto lab conducted the animal and biochemical work, while chemists Jun Yang and Sung Hee Hwang of the Hammock lab performed the chemistry and analytical chemistry. Takeo Yoshikawa, a team leader with the RIKEN's Molecular Psychiatry Laboratory, performed measurements of gene expression in the neurospheres from iPSC (induced pluripotent stem cells) from schizophrenia patients and postmortem brain samples from autism patients.

Exciting and Productive
Hashimoto described the international collaboration as “exciting and productive.” This is their third PNAS paper in a series leading to endoplasmic reticulum stress. “We report discovery of a biochemical axis that leads to multiple neurological disorders, including depression, Parkinson's disease, schizophrenia, autism spectrum disorders and similar diseases,” he said.

William Schmidt, vice president of clinical development at EicOsis, a Davis-based company developing inhibitors to sEH to treat unmet medical needs in humans and companion animals, said the company is devel​oping a first-in-class therapy for neuropathic and inflammatory pain. “EicOsis is in the process of finalizing our first human trials on the inhibitors of the soluble epoxide hydrolase, originally reported from UC Davis,” Schmidt said. “We are targeting the compounds as opioid replacements to treat peripheral neuropathic pain. It is exciting that the same compound series may be used to prevent or treat diseases of the central nervous system.”

Several grants from the National Institutes of Health, awarded to Hammock, supported the research. Hammock praised the many collaborators and students he has worked with on the project. “This work illustrates the value of research universities in bringing together the diverse talent needed to address complex problems,” Hammock said. “It also illustrates the value of fundamental science. This autism research can be traced directly to the fundamental question of how caterpillars turn into butterflies.”

Identification. Collaboration. Camaraderie.

The scientists and butterfly/moth enthusiasts who gathered Saturday, Feb. 9 for the Northern California Lepidoptera  Society meeting in the Bohart Museum of Entomology at the University of California, Davis, did all that: identify specimens and engage in collaboration and camaraderie.

They ranged from those early in their career, to mid-point, to the height of their career, to retired.

The group meets for a mid-winter gathering once a year, alternating between the Bohart Museum of Entomology at UC Davis and the Essig Museum of Entomology at UC Berkeley.

Senior museum scientist Steve Heydon, Bohart associate John "Moth Man" DeBenedictis; and Jeff Smith, curator of the butterfly/moth section at the Bohart hosted the event, assisted by Lynn Kimsey, director of the Bohart Museum and Bohart associate and naturalist Greg Kareofelas. 

In their meeting notice, Heydon, DeBenedictis and Smith noted that those attending could bring specimens, photos, PowerPoint presentations "or slides from collecting trips and tales of collecting triumphs to share with others
and that "attending lepidopterists may be able to help you identify specimens and the museum collection will be open for yoour inspection."

Christopher Jason, a recent UC Davis graduate in environmental science, was there with his collection. In March he will head to Washington State University, the Vancouver, campus, to work as a technician in the lab of conservation biologist/associate professor Cheryl Schultz, who researches at-risk butterflies in Pacific Northwest Prariries. Or, as Schultz. writes on her website: "Human-caused changes in the earth's ecosystems are responsible for the decline and extinction of the world's biological diversity. In the Conservation Biology Research Group, we study the ecology of at-risk species in response to key drivers: habitat loss, habitat fragmentation, habitat degradation, invasive species, alteration of ecosystem process, and global climate change. Using a population-ecology lens, we use field and quantitative methods to address pivotal ecological questions. We seek to understand the relative contributions of individual, population and landscape-level processes to the population viability of endangered species as these processes interface with realistic conservation interventions. Our work largely uses at-risk butterflies in Pacific Northwest Prairies as a model system to address fundamental ecological questions while simultaneously tackling focal questions to guide on-the-ground practice."

Retired public health entomologist and Bohart associate Dick Meyer of Bakersfield, known as "the mosquito guy," was there. He recently retired as assistant manager of the Orange County Vector Control Agency. He did not bring any of his collection, but he did tell us that he has 225 drawers of insects at home, including 71 drawers of butterflies. "Did you know that the highest diversity of butterflies in the country is in Kern County?" he asked. Meyer, who holds a doctorate in entomology, studied with major professor Richard M. Bohart Jr., for whom the Bohart Museum is named.

Among those participating:

• Bohart associate Jerry Powell, emeritus director of the Essig Museum, and co-author of California Insects.
• Marc Epstein, senior insect biosystematist for the California Department of Food and Agriculture and author of the book, Moths, Myths and Mosquitoes: the Eccentric life of Harrison G. Dyer Jr.
• Kelly Richers, treasurer of the Lepitopterists Society and an affiliate of Essig Museum of Entomology who is also  afield associate with the Los Angeles County Museum of Natural History and member of board of directors of the Wedge Entomological Research Foundation
• Lawrence "Larry" Allen of Calaveras County, author of A Field Guide to the West Coast Butterflies of the United States, one of the books available in the Bohart Museum's gift shop (he donated all sales of his book that day to the Bohart).
• Arthur Shapiro, UC Davis distinguished professor of evolution and ecology and author of Field Guide to Butterflies of the San Francisco Bay Area and Sacramento Valley Regions.
• Entomologist Rick Kelson, who directs the butterfly habitat at Six Flags Discovery Kingdom, Vallejo
• Bohart associate Bill Patterson, former graduate student of Richard Bohart
• Rosser Garrison, research associate with the California State Collection of Arthropods, retired senior insect biosystematist, California Department of Food and Agriculture, and co-author of Dragonfly Genera of the New World: An Illustrated and Annotated Key to the Anisoptera
• Don Miller, professor at Chico State University who teaches entomology in the Department of Biological Sciences
• Ryan Hill, professor at University of Pacific, Stockton
• Chris Tenney,  retired educator from Pacific Grove
• Jeffrey Caldwell, known for his ecological restoration
• John Lane, one of first graduate students of Art Shapiro (Lane received his master's degree)
• Paul Johnson, biologist with the National Parks Service
• Hobbyist Jeff Baier of Napa
• Physician Val Albu of Fresno
• And many more...

At the last gathering in the Bohart, Kelly Richers, who compiles the California Moth Specimen Database, maintained at the Essig Museum since 1996 as a resource to better survey and understand California moths, said of the systematists:  "We're a dying breed." 

This year self-described "aspiring entomologist" Madison Cunha of Modesto attended with her mother, Christine Cunha. True to her love of insects, Madison wore a dress adorned with a beetle pattern.

Scientists say that 180,000 species of the Lepidoptera are described, in 126 families and 46 superfamilies.

"The Lepidoptera are among the most successful groups of insects. They are found on all continents, except Antarctica, and inhabit all terrestrial habitats ranging from desert to rainforest, from lowland grasslands to mountain plateaus, but almost always associated with higher plants, especially angiosperms (flowering plants). Among the most northern dwelling species of butterflies and moths is the Arctic Apollo (Parnassius arcticus), which is found in the Arctic Circle in northeastern Yakutia, at an altitude of 1500 m above sea level. In the Himalayas, various Apollo species such as Parnassius epaphus have been recorded to occur up to an altitude of 6,000 miles above sea level."--Wikipedia.

Oh, the butterflies you'll see at the Bohart Museum of Entomology during the eighth annual UC Davis Biodiversity Museum Day on Saturday, Feb. 16.

Entomologist Jeff Smith, who curates the Lepidoptera (butterflies and moths) collection, says "I believe we have half a million Lepitoptera in our collection, and it is likely 60 percent moths and 40 percent butterflies."

What can visitors expect to see at the Bohart Museum during the four-hour open house from 9 to 1 p.m. on Biodiversity Museum Day? The Bohart will be one of 13 museums or collections open to the public and the event if free and family friendly.

"They will see thousands of species of butterflies--and maybe moths if we open those aisles as well--that they have never seen before," Smith says. "They can learn about the defensive strategies these insects use for survival, such as camouflage, warning coloration, mimicry of other species. We love to teach about the importance of Lepidoptera in the environment, either to their habitat directly or possibly as an indicator of the health of their habitat. And, of course, they can be amazed at the beauty of these insects."

This is truly a global collection.

"We have specimens from all over the world, from every continent," Smith notes. "We are especially strong, of course, in North American material but also strong in Central and South America, and these areas represent much of our continuing growth. We have a great deal of material from Papua New Guinea, Australia, Indonesia, Malaysia, Taiwan, and other eastern Asian countries. We have a great amount of material from Africa, particularly Zambia and Democratic Republic of the Congo. We are especially strong with northern Mexico moths, much of which likely represents species still undescribed by taxonomists."

Are endangered or threatened butterflies in the collection? Yes.

"We do have examples of most of the threatened and endangered species of butterflies, and even examples of some of the butterfly species now extinct (due to destruction of habitat by urbanization). Due to wonderful donations over the decades we have examples of very rare species from Asia and Africa, as well as a growing collection of the extraordinary Birdwing Butterflies from the Indonesian-Australian region."

The Bohart's monarch butterfly specimens comprise five drawers and they include specimens from the Pacific Islands, Australia and Eastern Asia, as well as the United States.

"Without a doubt, the biggest 'Wow Factor' comes from the huge and brilliant metallic blue Morpho butterflies from tropical America," Smith points out. "We also have four drawers of the Atlas moths from Asia, the largest insects in the world. We have specimens of the White Witch moth, with the widest wingspan of any Lepidoptera. We have many drawers of Asian swallowtails that are brilliant metallic blue and green, as well as the striking Birdwing Butterflies that are green, blue, gold, and HUGE."

Smith himself has collected many of them. "I have not kept track, but I have been passionate about Lepidoptera since 1967, have made 12 research trips to Latin America, and probably have collected 50,000 to 60,000 specimens myself, all of which reside in the Bohart collection."

Does he have a favorite?

"Oddly, my favorite group of butterflies may be the Arctics (genus Oeneis), which occur throughout the western United States, Canada, and to Alaska. I like them due to their habitat, normally, of high mountain areas, and they remind me of the thrill I feel when hiking in these areas. My favorite moths could be the wasp-mimics in the Arctiid moths, the subfamily Ctenuchinae. Hundreds of different species are found in the Neo-Tropics and their amazing colors and often strong resemblance to wasps intrigues me."

Smith, a resident of Rocklin, received a 2015 “Friend of the College” award from the UC Davis College of Agricultural and Environmental Sciences for his volunteer work, including preparing specimens and organizing the  collection. At the time he had spread the wings of more than 200,000 butterflies and moths, amounting to 33,000 hours of volunteer service.

Today it's many more. Many, many more.

“Entomology is my passion," he says, "and the Bohart Museum is my cause.”

Lynn Kimsey, director of the Bohart Museum and UC Davis professor of entomology, who nominated Smith for the award, praised him for completely reorganizing the butterfly and moth collection. “It's no small feat to rearrange this many specimens, housed in roughly one thousand drawers,” she said. “Many thousands of the specimens needed to be identified, and the taxonomy required extensive updating and reorganization.”

In her nomination letter, she wrote that “You could not ask for a better friend than Jeff Smith. He has brought us international acclaim and saved us $160,000 through donations of specimens and materials, identification skills and his professional woodworking skills. This does not include the thousands of hours he has donated in outreach programs that draw attention to the museum, the college and the university.”

Kimsey, who has directed the museum since 1989, remembers when Smith joined the museum. “When Jeff was working for Univar Environmental Services, a 35-year career until his retirement in 2013, he would spend some of his vacation days at the museum. Over the years Jeff took over more and more of the curation of the butterfly and moth collection. He took home literally thousands of field pinned specimens and spread their wings at home, bringing them back to the museum perfectly mounted."

The Bohart Museum houses a global collection of nearly eight million specimens. It is also the home of the seventh largest insect collection in North America, and the California Insect Survey, a storehouse of the insect biodiversity. Noted entomologist Richard M. Bohart (1913-2007) founded the museum.

Special attractions include a “live” petting zoo, featuring Madagascar hissing cockroaches, walking sticks and tarantulas. Visitors are invited to hold the insects and photograph them. The museum's gift shop, open year around, includes T-shirts, sweatshirts, books, jewelry, posters, insect-collecting equipment and insect-themed candy.

The Bohart Museum's regular hours are from 9 a.m. to noon and 1 to 5 p.m. Mondays through Thursdays. The museum is closed to the public on Fridays and on major holidays. Admission is free. Open houses, focusing on specific themes, are held on weekends throughout the academic year. More information on the Bohart Museum is available by contacting (530) 752-0493 or Tabatha Yang, education and public outreach coordinator at tabyang@ucdavis.edu

UC Davis Biodiversity Museum Day
The UC Davis Biodiversity Museum Day, always held the Saturday of Presidents' Day weekend, is billed as a “free, educational event for the community where visitors get to meet and talk with UC Davis scientists from undergraduate students to staff to emeritus professors and see amazing objects and organisms from the world around us.” Parking is also free. Maps are available on the Biodiversity Museum Day website at http://biodiversitymuseumday.ucdavis.edu/.

These seven collections will be open from 9 a.m. to 1 p.m.:

• Arboretum and Public Garden, Good Life Garden, next to the Robert Mondavi Institute, 392 Old Davis Road, on campus
• Bohart Museum of Entomology, Room 1124 of the Academic Surge Building, Crocker Lane
• California Raptor Center, 340 Equine Lane, off Old Davis Road
• Museum of Wildlife and Fish Biology, Room 1394, Academic Surge Building, Crocker Lane
• Paleontology Collection, Earth and Physical Sciences Building, 434 LaRue Road
• Phaff Yeast Culture Collection, Robert Mondavi Institute of Wine and Food Science, 392 Old Davis Road, on campus
• Viticulture and Enology Culture Collection, Robert Mondavi Institute of Wine and Food Science, 392 Old Davis Road, on campus

 The following will be open from noon to 4 p.m.:

• Anthropology Museum, 328 Young Hall and grounds
• Botanical Conservatory, Greenhouses along Kleiber Hall Drive
• Center for Plant Diversity, Sciences Laboratory Building, off Kleiber Hall Drive
• Häagen-Dazs Honey Bee Haven, Bee Biology Road, off Hopkins Road (take West Hutchison Drive to Hopkins)
• Nematode Collection, Sciences Laboratory Building, off Kleiber Hall Drive
• Marine Invertebrate Collection, Sciences Laboratory Building, off Kleiber Hall Drive