Doctoral candidate Lindsey Mack

Infected Ae. aegypti mosquitoes can transmit dengue fever, chikungunya, Zika fever, Mayaro and yellow fever viruses, and other disease agents.

Mack will present her exit seminar on "Genetic and Molecular Factors Influencing Pyrethroid Response in Aedes aegypti from California" at 3:30 p.m., Tuesday, June 6 in 366 Briggs Hall. It also will be on Zoom. 

Mack studies Ae. aegypti with a focus on analysis of transcriptomic datasets and 3D imaging datasets. "Throughout my time in graduate school, my projects have considered pyrethroid resistance in Ae. aegypti; examining the genetic response to this insecticide. As I finish up my dissertation, I hope to pursue a career in industry using the skills I've developed to continue to analyze large datasets!"

Insecticide resistance is a global issue, Mack says in her exit seminar abstract. The mosquito was first colonized California in 2013 and arrived resistant to pyrethroids. "The pyrethroid target site genotype differs geographically in California and partially infers resistance phenotype, indicating that other mechanisms are at play as well."

Mack is the co-lead author (with doctoral candidate Erin Taylor Kelly of the Attardo lab) of  Frequency of Sodium Channel Genotypes and Association with Pyrethrum Knockdown Time in Populations of Californian Aedes aegypti, published in March 2021 in the journal Parasites and Vectors. The eight co-authors, in additioin to Attardo, included Anthony Cornel, Mosquito Control Research Laboratory, Kearney Agricultural Center, and Department of Entomology and Nematology. 

 "Since their detection in 2013, Aedes aegypti has become a widespread urban pest in California," the co-authors wrote in the abstract. "The availability of cryptic larval breeding sites in residential areas and resistance to insecticides pose significant challenges to control efforts. Resistance to pyrethroids is largely attributed to mutations in the voltage gated sodium channels (VGSC), the pyrethroid site of action. However, past studies have indicated that VGSC mutations may not be entirely predictive of the observed resistance phenotype."

"To investigate the frequencies of VGSC mutations and the relationship with pyrethroid insecticide resistance in California, we sampled Ae. aegypti from four locations in the Central Valley, and the Greater Los Angeles area. Mosquitoes from each location were subjected to an individual pyrethrum bottle bioassay to determine knockdown times. A subset of assayed mosquitoes from each location was then analyzed to determine the composition of 5 single nucleotide polymorphism (SNP) loci within the VGSC gene."

The conclusion:

"Resistance associated VGSC SNPs are prevalent, particularly in the Central Valley. Interestingly, among mosquitoes carrying all 4 resistance associated SNPs, we observe significant heterogeneity in bottle bioassay profiles suggesting that other mechanisms are important to the individual resistance of Ae. aegypti in California."

Mack,  who holds a bachelor of science degree (2018) in biology from Creighton University, Omaha, Neb.,  enrolled in the UC Davis graduate school program in 2018. 

Active in the Entomological Society of America, Mack scored second place in student competition at the 2022 joint meeting of the Entomological Societies of America, Canada, and British Columbia, held last November in Vancouver, British Columbia. She entered her presentation, "Three Dimensional Analysis of Vitellogenesis in Aedes aegypi Using Synchrotron X-Ray MicroCT,” in the category, "Graduate School Physiology, Biochemistry and Toxicology: Physiology.

Her abstract: "Traditional methods of viewing the internal anatomy of insects require some degree of tissue manipulation and/or destruction. Using synchrotron-based x-ray phase contrast microCT (pcMicroCT) avoids this issue and has the capability to produce high contrast, three dimensional images. Our lab is using this technique to study the morphological changes occurring in the mosquito Aedes aegypti during its reproductive cycle. Ae. aegypti is the primary global arbovirus vector, present on all continents except Antarctica. Their ability to spread these viruses is tightly linked with their ability to reproduce, as the production of eggs in this species is initiated by blood feeding. Amazingly, this species produces a full cohort of eggs (typically 50-100) in just 3 days' time following a blood meal. This rapid development represents dramatic shifts in physiological processes that result in massive volumetric changes to internal anatomy over time. To explore these changes thoroughly, a time course of microCT scans were completed over the vitellogenic period. This dataset provides a virtual representation of the volumetric, conformational, and positional changes occurring in tissues important for reproduction across the vitellogenic period. This dataset provides the field of vector biology with a detailed three-dimensional internal atlas of the processes of vitellogenesis in Ae. aegypti."

"As for career plans, I am applying to computational biology positions in industry," Mack said. "I'm not filing my dissertation until July so I am still working on this."

Time flies but how do flies tell time? (Photo by Kathy Keatley Garvey)

That was molecular geneticist Yao Cai, entertaining with several other members of the UC Davis Entomology Graduate Student Association (EGSA). He was dressed--quite appropriately, too--as a fruit fly, Drosophila melanogaster, which he described as “our favorite model organism in Insecta!” (See Bug Squad blog.)

Entomology Today, a publication of the Entomological Society of America, picked up the story, headlining it as "Bugs and Beats."

Now fast forward...past the two-year pandemic...and way over to 2022. Time flies, right?

Cai now holds a doctorate in entomology (as of September) and will present his exit seminar, "How Do Flies Tell the Time of Day?" at the next UC Davis Department of Entomology and Nematology seminar, set Wednesday, Oct. 26.

He will deliver his seminar both in-person and virtually at 4:10 p.m. in 122 Briggs Hall. The Zoom link:
https://ucdavis.zoom.us/j/95882849672.

Cai will be introduced by his major professor, molecular geneticist and physiologist Joanna Chiu, professor and vice chair of the department. 

A fruit fly on a raspberry. This is a spotted wing drosophila from the Joanna Chiu lab at UC Davis. (Photo by Kathy Keatley Garvey)

"This study highlights the importance of post-translational regulation of circadian rhythms," Cao noted. "Finally, together with previous studies in fungi and mammals, our results suggest a conserved feature in eukaryotic clocks by which transcriptional repressors recruit CK1s to modulate the activity of transcription activators."

A native of southeast Asia, Cai holds two degrees from China Agricultural University, Beijing: a bachelor of science degree (2014) in plant protection and a master's degree in entomology (2016).

What sparked his interest in entomology? "The insect world presented to me the diversity of species when I was a kid," he related. "Since then, I have wondered about the origin and evolution of species. I was lucky to cultivate my interest as an undergrad and then a master student in Chinese Agricultural University. As a PhD student in the Department of Entomology andNematology at UC Davis, this interest expanded to the cellular and molecular mechanisms of evolution and adaptation. Upon my graduation in summer 2022, I continued my postdoctoral research in the Chiu lab. I hope this will prepare me to become a professor in biological sciences."

Cai completed an International Chronobiology Summer School (virtual) in 2020 and a UC Davis Comprehensive Course in Flow Cytometry in 2019. His publications include: 

• Cai YD*, Joshi R*, Xia Y, Chiu JC, Emery P (2022) PERIOD phosphoclusters control temperature compensation of the Drosophila circadian clock, Frontiers in Physiology. 13: 888262.
  
  
• Cai YD, Sotelo SH, Jackson K, Chiu JC (2022) Assaying circadian locomotor activity rhythm in Drosophila, in “Circadian Rhythms” in NeuroMethods series. ed. M. Hatori, T. Hirota, and S. Panda. Springer Nature, Switzerland.
  
  
• Cai Y.D., Y. Xue, C. Truong, J. Del-Carmen Li, C. Ochoa, J.T. Vanselow, K.A. Murphy, Y.H. Li, X. Liu, B.L. Kunimoto, H. Zheng, C. Zhao, Y. Zhang, A. Schlosser, J.C. Chiu (2021). CK2 inhibits TIMELESS nuclear export and modulates CLOCK transcriptional activity to regulate circadian rhythms. Current Biology 31(3): 502-514.
  
  
• Cai, Y.D., J.C. Chiu (2021). Timeless in animal circadian clocks and beyond. FEBS Journal (Online ahead of print). doi: 10.1111/febs.16253.
  
  
• Abrieux, A., Y. Xue, Y. Cai, K.M. Lewald, H.N. Nguyen, Y. Zhang, J.C. Chiu. (2020). EYES ABSENT and TIMELESS integrate photoperiodic and temperature cues to regulate seasonal physiology in Drosophila. Proceedings of the National Academy of Sciences. U.S.A. 117(26): 15293-15304. 

Active in academics, Cai served as a guest lecturer for Entomology 102 on the "Insect Nervous System" and for Entomology 10 on "Insect Circadian Rhythm." He also served as a teaching assistant for a number of UC Davis classes, including Applications, Values, and Ethics in Animal Research; Insect Physiology; Introduction to Biology: Ecology and Evolution; Introduction to Biology: Cell Functions; and Calculus for Biology and Medicine. In addition, he has assisted at Bohart Museum of Entomology open houses and at science program provided by Peregrine School, Davis.

Honored with a number of awards, Cai received a 2021 Chinese Government Award for Outstanding Self-Finance Students Abroad; a 2021 UC Davis Entomology W. Harry Lange, Jr. Memorial Travel Fund; 2021 UC Davis Marv Kinsey Scholarship; a 2020 Boroughs Welcome Fund Society for Research on Biological Rhythms (SRBR) Excellence Award; a 2020 UC Davis Sean and Anne Duffey and Hugh and Geraldine Dingle Research Fellowship; a 2019 UC Davis McBeth Memorial Scholarship; and a 2018 and 2017 UC Davis Henry A. Jastro Graduate Research Awards, among others.

Emily Meineke, assistant professor of urban landscape entomology, UC Davis Department of Entomology and Nematology, coordinates the department's seminars for the 2022-23 academic year. All 11 seminars will take place both person and virtually at 4:10 p.m. on Wednesdays in Room 122 of Briggs Hall except for the Nov. 9th and Dec. 7th seminars, which will be virtual only, she said.  (See list of seminars)

For further information on the seminars or to resolve any technical difficulties with Zoom, contact Meineke at ekmeineke@ucdavis.edu. 

UC Davis doctoral candidate Olivia Winokur will present her exit seminar Oct. 5.

Winokur, a UC Davis doctoral candidate and vector-borne disease specialist who studies with major professor Chris Barker, will present her exit seminar, hosted by the UC Davis Department of Entomology and Nematology, at 4:10 p.m., Wednesday, Oct. 5 in 122 Briggs Hall.

She'll discuss "Temperature Drives Transmission of Mosquito-borne Pathogens: Improving Entomological Estimates for Aedes aegypti-borne Virus Transmission Risk." Her seminar will be both in-person and virtual. The Zoom link: https://ucdavis.zoom.us/j/95882849672.

"The mosquito Aedes aegypti is the primary vector of a range of viruses that cause a major burden on human health worldwide, including dengue, Zika, chikungunya, and yellow fever viruses," Winokur writes in her abstract. "As the Zika epidemic emerged in 2016, estimates for Zika risk were based on proxy evidence from closely related dengue virus. To improve risk estimates, we studied how temperature affects Zika virus extrinsic incubation period. We sought to further improve risk estimates by studying thermal preferences of Ae. aegypti mosquitoes in the laboratory and in the field. Current mosquito-borne pathogen risk models primarily use temperatures from weather stations or thermal imagery as a proxy for the temperatures mosquitoes experience, however such approaches do not account for local environments or microclimates available to adult mosquitoes. Taken together, the results of these studies can be used to improve prediction of mosquito-borne pathogen risk and inform mosquito control decisions." (See information on the mosquito on the California Department of Public Health website)

The yellow fever mosquito, Aedes aegypti. (CDC Photo)

Olivia received her bachelor's degree in May of 2015 from Cornell University where she was an interdisciplinary studies major (environmental effects on human health).

At UC Davis, Winokur served as the 2019-2020 president of the Entomology Graduate Student Association and as a 2020-2022 committee member of the UC Davis Entomology Diversity, Equity, Inclusion, & Belonging. She co-founded the Girls' Outdoor Adventure in Leadership and Science (GOALS) in 2017 and continues to serve in leadership roles. GOALS is a free two-week summer science program for high school girls and gender expansive youth from backgrounds underrepresented in STEM fields. They learn science, outdoors skills, and leadership hands-on while backpacking in Sequoia National Park.

Some of Olivia Winokur-authored research

Active in the vector-borne disease community, Winokur completed a 2019-2020 term as the Executive Council student representative for the American Committee on Medical Entomology (ACME) and as a 2017-2019 volunteer with the Vector-Borne Disease Section of the California Department of Public Health, where she assisted with hantavirus and plague surveillance.   She peer-reviews manuscripts for the Journal of Medical Entomology.

Winokur is the recipient of numerous fellowships and grants, including a $140,00 National Science Foundation Graduate Research Fellowship; a $30,000 Pacific Southwest Center of Excellence in Vector-Borne Diseases Graduate Fellowship;   a $25,000 Pacific Southwest Center of Excellence in Vector-Borne Diseases Postdoctoral Fellowship; a Professors for the Future Fellowship (UC Davis) of $3,000; and two-consecutive Hazeltine Student Research Awards (UC Davis), totaling  $5,500. She also received an American Geophysical Union Centennial Grant of $9,720 and an American Association for University Women Community Action Grant of $5,000 (outreach grants).

Winokur's latest peer-reviewed publications include co-authoring "The Influence of Vector-borne Disease on Human History: Socio-Ecological Mechanisms" in the journal Ecology Letters; and serving as the lead author of "Impact of Temperature on the Extrinsic Incubation Period of Zika Virus in Aedes aegypti in the journal PLOS (Public Library of Science): Neglected Tropical Diseases.

Emily Meineke, assistant professor of urban landscape entomology, UC Davis Department of Entomology and Nematology, coordinates the department's seminars for the 2022-23 academic year. All 11 seminars will take place both person and virtually at 4:10 p.m. on Wednesdays in Room 122 of Briggs Hall except for the Nov. 9th and Dec. 7th seminars, which will be virtual only, she said.  (See list of seminars)

For further information on the seminars or technical difficulties with Zoom, contact Meineke at ekmeineke@ucdavis.edu. 

Hanna Kahl working in the field.

 “They feed mostly at night and hide during the day,” according to the UC Statewide Integrated Pest Management Program. They hide in the bark crevices, mulch, and “protected compact plant parts such as dried, curled leaves, and under trunk wraps.” 

“Earwigs feed on dead and living insects and insect eggs and on succulent plant parts, UC IPM says. "Earwig nymphs and adults will climb trees and feed on flower buds, leaves, and fruit of trees during the spring flush months (March through May). Nymphs tend to feed on plant material more than adults...Earwigs can be very problematic on young trees with trunk wraps or cardboard guards, in which they reside. They climb the trees and feed on the new leaves. Large numbers of earwigs can defoliate trees.”

Enter ecological pest management specialist Hanna Kahl, who recently received her doctorate in entomology from UC Davis, studying with UC Davis distinguished professor Jay Rosenheim. She'll present her exit seminar on “Herbivory of Citrus Fruit by European Earwigs in California” at 4:10 p.m, Wednesday, Sept. 29 in Room 122 of Briggs Hall. This in-person will be the first of the UC Davis Department of Entomology and Nematology's fall seminars. Many of the others will be virtual. 

“Growers and pest control advisors in California suspect that European earwigs (Forficula auricularia) damage young citrus fruit,” Kahl writes in her abstract. “However, very little is known about herbivory by earwigs on citrus fruit. Our work details characteristics of herbivory by earwigs on citrus fruit and the use of sticky and pesticide barriers to manage earwigs and other citrus pests.” 

Kahl, awarded her doctorate in August, focused her research on understanding the role of European earwigs in California citrus; developing a whole systems approach to manage earwigs and other citrus pests; and feeding preferences of fort-tailed bush katydids and citrus thrips on California citrus. 

Kahl is now an ecological pest management specialist at Community Alliance with Family Farmers, Davis. She leads projects and extension efforts on sustainable pest management tactics. 

She received an ongoing grant in 2019 from the Citrus Research Board on “Characterizing Earwig Damage to Citrus Fruits, and Damage Prevention using Trunk Barrier Treatment.” She also received a National Science Foundation Graduate Research Fellowship, awarded in 2017, and a 2018-19 Keller Pathways Fellowship (for entrepreneurship) from the University of California.

Active in both the Entomological Society of America and the Ecological Society of America, she won first place in the Student 15-Minute Paper Competition at the 2016 meeting of the International Congress of Entomology; and second place in the Student 10-Minute Paper Competition at the 2019 meeting of the Entomological Society of America (ESA). She served on the Eastern Branch team that won second place in the ESA's 2016 Linnaean Games, now called the Entomology Games. She co-organized  a seminar, “Agroecology with Communities: Cross-Disciplinary Collaborations Between Ecology, Agriculture and Social Science,” for the Ecological Society of America at its 2019 meeting. 

Kahl received her bachelor's degree in biology from Whitman College, Walla Walla; her master's degree in entomology from the University of Maryland, College Park. At Whitman College, she researched the effects of reservoirs as habitat barriers on song sharing of the birds, dickcissels. At the University of Maryland,  she focused her research topics on effects of red clover as a living mulch cover crop on arthropod herbivores, natural enemies, pollination and yield in cucumber; (2) consumptive and non-consumptive effects of wolf spiders on cucumber beetles and (3) effects of red clover living mulch on greenhouse gas emissions.  She studied abroad in a six-month School for International Training program in Jaipur, Rajasthan, India in 2010. Her research topic: Village dairy production in Haryana and Orissa.

Her current activities include being the chapter leader for Women in Data (WID), Sacramento. 

Kahl's exit seminar will be recorded for later viewing, according to seminar coordinator Shahid Siddique (ssiddique@ucdavis.edu), a nematologist and assistant professor, UC Davis Department of Entomology and Nematology. The seminars take place every Wednesday at 4:10 p.m. Many are virtual.