Posts Tagged: yield
Don't wait too long to irrigate your wheat: maintaining yield potential in this winter's dry period
Long dry periods are not uncommon in the Sacramento Valley between January and February. Despite...
UC Davis team identifies wheat gene that increases yield
The findings could help growers produce more wheat without expanding operation
A team of scientists from University of California, Davis, have identified a new gene variant in wheat that can increase the amount of the grain produced, new research published in the journal PLOS Genetics finds.
Wheat is a staple of food diets worldwide and the gene discovery could allow farmers to grow more food without increasing land use. Increased yield could also lower consumer prices, making the crop more accessible.
“We have a growing human population that likes to eat every day,” said Jorge Dubcovsky, a plant sciences distinguished professor who led the research. “We need to produce more wheat in the same space so we need plants that are more productive.”
The researchers found a gene – WAPO1 – that controls the maximum number of grains in a wheat spike. Breeding the beneficial gene variant into the plants could delay the formation of the terminal spikelet, providing room for more grains to grow in each spike rather than ending production of grain.
WAPO1 is one of the first genes discovered that can affect wheat yield. “We are trying to make more productive wheat varieties and we are starting to understand how that trait is controlled,” Dubcovsky said.
Pasta wheat lacking the gene
The gene variant for high grain number is found frequently in bread wheats but not in pasta wheats. By breeding the beneficial gene variant into those pasta wheat varieties, growers could increase yield by 4% to 5% in cultivars that have the biomass capacity to fill the extra grains.
“We developed molecular markers to select for the form of that gene to produce increased yield,” Dubcovsky said. “It's a significant step forward.”
Previous research by the team mapped the gene and identified others that could affect yield. This research confirmed those findings for WAPO1.
Discovery on path to future yield increases
The WAPO1 gene is part of a network of genes that work together to control yield, and researchers need to identify the best variant combinations to maximize yield. Solving this puzzle can lead to better production rates.
“We will continue to try to understand the network of genes that control the yield of wheat,” he said.
Saarah Kuzay, Huiqiong Lin, Chengxia Li, Shisheng Chen, Daniel P. Woods and Junli Zhang from UC Davis also contributed to the research, as did scientists from Howard Hughes Medical Institute, Heinrich Heine University and Peking University Institute of Advanced Agricultural Sciences.
Funding was provided by USDAs National Institute of Food and Agriculture's Food Research Initiative, the International Wheat Yield Partnership and Howard Hughes Medical Institute.
/h3>/h3>/h2>How wildfire restored a Yosemite watershed
Reposted from Berkeley News
For nearly half a century, lightning-sparked blazes in Yosemite's Illilouette Creek Basin have rippled across the landscape — closely monitored, but largely unchecked. Their flames might explode into plumes of heat that burn whole hillsides at once, or sit smoldering in the underbrush for months.
The result is approximately 60 square miles of forest that look remarkably different from other parts of the Sierra Nevada: Instead of dense, wall-to-wall tree cover — the outcome of more than a century of fire suppression — the landscape is broken up by patches of grassland, shrubland and wet meadows filled with wildflowers more abundant than in other parts of the forest. These gaps in the canopy are often punctuated by the blackened husks of burned trunks or the fresh green of young pines.
“It really is a glimpse into what the Sierra Nevada was like 200 years ago,” said Scott Stephens, a professor of environmental science, policy and management at the University of California, Berkeley, and co-director of Berkeley Forests.
Stephens is the senior author of a new study that gathers together decades of research documenting how the return of wildfire has shaped the ecology of Yosemite National Park's Illilouette Creek Basin and Sequoia and Kings Canyon National Parks' Sugarloaf Creek Basin since the parks adopted policies for the basins — at Illilouette Creek in 1972 and Sugarloaf Creek in 1968 — to allow lightning-ignited fires to burn.
While the prospect of smoke over iconic Half Dome has worried politicians and tourists alike, the work of Stephens and his colleagues demonstrates that allowing frequent fires to burn in these basins has brought undeniable ecological benefits, including boosting plant and pollinator biodiversity, limiting the severity of wildfires and increasing the amount of water available during periods of drought. All these benefits are also likely to make the forest more resilient to the warmer, drier conditions brought by climate change, the research suggests.
“In many ways, fire has successfully been restored to Illilouette, and it has made for a complex mosaic of vegetation with cascading effects on things like water,” said study co-author Brandon Collins, who holds a joint appointment as a research scientist with Berkeley Forests and with the U.S. Forest Service. “In Illilouette, you can have patches of young, regenerating trees from a fire 15 years ago, or areas where a classic understory burn has resulted in big, old, widely-spaced trees. You can even have areas where fire has missed because there's more moisture, such as adjacent to a creek or on the edge of a meadow. All this complexity can happen in a really short amount of space.”
The study findings arrive in the middle of a critical fire season, when drought conditions throughout the western U.S. have already sparked numerous large wildfires, including the Dixie Fire, which, as of Aug. 8, was the second largest wildfire in California history. While climate change has played a role in increasing the severity of these fires, Stephens said, Illilouette Creek Basin serves as an example of how current forest conditions in the Sierra — largely shaped by decades of fire suppression — are also driving these massive blazes.
“I think climate change is no more than 20 to 25% responsible for our current fire problems in the state, and most of it is due to the way our forests are,” Stephens said. “Illilouette Basin is one of the few places in the state that actually provides that information, because there is no evidence of changes in fire size or in the severity of fires that burn in the area. So, even though the ecosystem is being impacted by climate change, its feedbacks are so profound that it's not changing the fire regime at all.”
Returning fire to Yosemite
For millennia, wildfires sparked by lightning, or lit by Native American tribes, regularly shaped the landscape of the western U.S., not only causing destruction, but also triggering necessary cycles of rebirth and regeneration. However, the arrival of European colonists in the late 1800s, followed by formation of the U.S. Forest Service in 1905, ushered in an era in which fire was viewed as the enemy of humans and forests alike, and the vast majority of wildfires were quickly extinguished.
By the 1940s and 1950s, a number of forest managers and ecologists had begun to question the wisdom of fire suppression, noting that the practice was eliminating valuable wildlife habitat and increasing the severity of fires by allowing decades of fuel buildup. These fire proponents included A. Starker Leopold, an acclaimed conservationist and professor of zoology and forestry at UC Berkeley, as well as Harold Biswell, a professor at UC Berkeley's School of Forestry.
In response to a foundational 1963 report led by Leopold, the U.S. National Park Service changed its policy in 1968 to allow lightning fires to burn within special fire management zones — usually remote regions at high elevations — where danger to human settlements was low. Sequoia and Kings Canyon National Parks established the first fire management zone in 1968, followed by Yosemite National Park in 1972.
“I think it was finally recognized that fire is an integral piece of these ecosystems, and there were a few key people who were willing to take the risk of letting these fires happen,” Collins said.
‘It isn't always clean, and it's not always nice'
Between 1973 and 2016, Illilouette Creek Basin experienced 21 fires larger than 40 hectares — approximately equal to 75 football fields — while Sugarloaf experienced 10 fires of that size. In Illilouette, the result today is a forest that may look a bit messy to the untrained eye, but it holds a lot of resilience.
“When some people visit Illilouette, they say, ‘Look at all these dead trees!'” Stephens said. “I think we have this idea that forests need to be green all the time and made up with only big trees. But it turns out that no forest can do that. It has to be able to grow young trees and regenerate. Illilouette is doing that, but it isn't always clean, and it's not always nice.”
In Illilouette, wildfire has created a more diverse array of habitats for animals like bees and bats, while allowing a variety of plant life to flourish. The detailed history of wildfires in Illilouette has also provided foresters with valuable information on how the impact of one wildfire on landscape and vegetation can influence the trajectory of the next wildfire.
“Since fires are generally allowed to burn freely in Illilouette, we could look at what happens when two fires have burned close to each other: When does the second fire burn into the area that was burned by the first fire, and when does it stop at the previous perimeter?” Collins said. “We found that it really depended on the amount of time that had passed since the first fire. If it had been nine years or under, fires almost never burned into a previous fire perimeter.”
Collins said that Illilouette has also given forest managers a unique opportunity to study how wildfire behaves under a variety of conditions, rather than only at its most dire.
“One of the things that's kind of perverse about the fire suppression policy is that we actually constrain fires to only burn under the worst conditions. If the fire is mellow, that's a good time to put it out, and, as a result, they only burn when we can't put them out,” Collins said. “But by letting these fires burn (in Illilouette), they're able to experience the full range of weather conditions. On bad days, some of these fires have really put up a pretty good plume. But on the flip side, they also get to burn under more moderate conditions, too, and it makes for really varied effects.”
Returning fire to Illilouette has also had the somewhat counterintuitive impact of increasing the availability of water in the basin, a key finding as California weathers yet another year of extreme drought.
Study co-author Gabrielle Boisramé, an assistant research professor at the Desert Research Institute in Nevada, began studying water in Illilouette as a Ph.D. student in environmental engineering at UC Berkeley. Her simulations and measurements indicate that small gaps in the tree canopy created by wildfires have allowed more water from snow and rainfall to reach the ground, while also reducing the number of trees competing for water resources. As a result, soil moisture in some locations in Illilouette increased as much as 30% between 1969 and 2012, which likely contributed to very low tree mortality in the basin during the drought years of 2014 and 2015.
Measurements also indicate that streamflow out of Illilouette Creek Basin has increased slightly since the managed wildfire program began, while streamflow out of other similar watersheds in the Sierras have all decreased. Boosting the amount of water that flows downstream is likely to benefit both the humans and the aquatic ecosystems that depend on this precious resource.
“There's more and more work being done that examines the effects of fire on hydrology, but most of the other research is looking at the effects of catastrophic fires that burned up an entire forest,” Boisramé said. “As far as we know, we're the only ones in the western U.S. studying a restored fire regime, where we're not just looking at one individual fire, but a number of fires of mixed severity that have occurred over natural intervals of time. There just aren't that many places to study the long-term effects of these repeated wildfires because Sugarloaf and Illilouette were the first areas in California — really the first western mountain watersheds — where they started allowing fires to burn most of the time.”
Fighting for fire
Most U.S. national parks now practice some form of fire use, rather than full fire suppression, and in 1974, the National Forest Service also changed its policy to also allow some fires to burn on its lands, although areas of fire use are rare in this agency. However, these federal fire use policies have struggled to gain a foothold, largely because of the inherent risks involved in managing wildfire.
Even in Sugarloaf Creek Basin, where many fires have been allowed to burn, there has also been significantly more fire suppression than in Illilouette, the study found. As a result, the ecological benefits in Sugarloaf are not as pronounced as those in Illilouette.
“I think one of the key things to recognize is that the landscape in Illilouette was already somewhat unique, partly because it is at slightly higher elevation than a lot of the forests we manage,” Collins said. “As a result, it already had a mix of vegetation with patches of meadows and rock, and I think maybe that gave managers a little more ease in letting fire happen there. It doesn't have the potential to really push off a giant megafire because it lacks the continuity that some of these other areas have.”
While both naturally-sparked fires and prescribed burns could help large swathes of the Sierra forest become more resilient to both drought and high severity fire, opposition to national “let it burn” policies in California remains strong, with state and local fire agencies often favoring the safety of fire suppression.
Collins and Stephens both acknowledge that the current fuel density in much of the Sierra, mixed with the hotter, drier conditions already triggered by climate change, has made managing wildfire even riskier than it was when forest managers started allowing fires to burn in Yosemite in 1972. However, they argue, fire suppression will never succeed in the long term, because the longer that forest fuel sources are allowed to build up, the more likely it becomes that wildfires will turn catastrophic when they are finally sparked.
“In order to actually allow this to happen, political and public institutions need to be willing to accommodate risk, because there will be some unpredictability. There are going to be fires that get larger, and more severe burning in places that have had very little fire for a century or more,” Stephens said. “We can't guarantee that Illilouette is going to be the new outcome, because it started when climate change was not nearly as severe. So, political institutions will have to accommodate that, or the first fire that doesn't do exactly what we hope will shut down the whole program.”
Collins and Stephens also advocate for more aggressive prescribed burning and restoration thinning throughout the Sierra to help get the forests to a place where lightning-sparked fires can be allowed to burn more safely.
Stephens credits strong, early leadership at Yosemite — including that of study co-author Jan W. van Wagtendok, who received a Ph.D. from UC Berkeley in 1972 and went on to serve as a research scientist at Yosemite for most of his career — for taking the huge risk of launching the program and allowing early fires to burn in the park.
“It's been 50 years now, but I think what we've learned helps us understand what is possible,” Stephens said. “We have 10 to 20 years to actually change the trajectory of the forest ecosystems in our state, and if we don't change them in 10 or 20 years, the forest ecosystems are going to change right in front of our eyes, and we're just going to be passengers. That's why it's so important to continue this work.”
Previous funding from the U.S. Joint Fire Science Program, UC ANR Competitive Grants Program, and the National Science Foundation's Critical Zone Collaborate Network (award number 2011346) supported the research in this paper.
Study co-authors also include Sally Thompson of the University of Western Australia; Lauren C. Ponisio of the University of Oregon, Eugene; Ekaterina Rakhmatulina, Jens Stevens and Zachary L. Steel of UC Berkeley; and Kate Wilkin of San Jose State University.
Impact of drip application of fungicides on strawberry health and yields
Strawberry, a high-value specialty crop in California, suffers from several soilborne, fruit, and foliar diseases. Verticillium wilt caused by Verticillium dahliae, Fusarium wilt caused by Fusarium oxysporum f. sp. fragariae, and Macrophomina crown rot or charcoal rot caused by Macrophomina phaseolina are major soilborne diseases that cause significant losses without proper control. Chemical fumigation, crop rotation with broccoli, nutrient and irrigation management to minimize plant stress, and non-chemical soil disinfestation are usual control strategies for these diseases. Botrytis fruit rot or gray mold caused by Botrytis cineaea is a common fruit disease requiring frequent fungicidal applications. Propagules of gray mold fungus survive in the soil and infect flowers and fruits. A study was conducted to evaluate the impact of drip application of various fungicides on improving strawberry health and enhancing fruit yields.
Methodology
This study was conducted in an experimental strawberry field at the Shafter Research Station during 2019-2020. Cultivar San Andreas was planted on 28 October 2019. No pre-plant fertilizer application was made in this non-fumigated field which had Fusarium wilt, Macrophomina crown rot, and Botrytis fruit rot in previous year's strawberry planting. Each treatment was applied to a 300' long bed with single drip tape in the center and two rows of strawberry plants. Sprinkler irrigation was provided immediately after planting along with drip irrigation, which was provided one or more times weekly as needed for the rest of the experimental period. Each bed was divided into six 30' long plots, representing replications, with an 18' buffer in between. Between 6 November 2019 and 9 May 2020, 1.88 qt of 20-10-0 (a combination of 32-0-0 urea ammonium nitrate and 10-34-0 ammonium phosphate) and 1.32 qt of potassium thiosulfate was applied 20 times at weekly intervals through fertigation. Treatments were applied either as a transplant dip or through the drip system using a Dosatron. The following treatments were evaluated in this study:
i) Untreated control: Neither transplants nor the planted crop was treated with any fungicides.
ii) Abound transplant dip: Transplants were dipped in 7 fl oz of Abound (azoxystrobin) fungicide in 100 gal of water for 4 min immediately prior to planting. Transplant dip in a fungicide is practiced by several growers to protect the crop from fungal diseases.
iii) Rhyme: Applied Rhyme (flutriafol) at 7 fl oz/ac immediately after and 30, 60, and 90 days after planting through the drip system.
iv) Velum Prime with Switch: Applied Velum Prime (fluopyram) at 6.5 fl oz/ac 14 and 28 days after planting followed by Switch 62.5 WG (cyprodinil + fludioxinil) at 14 oz/ac 42 days after planting through the drip system.
v) Rhyme with Switch: Four applications of Rhyme at 7 fl oz/ac were made 14, 28, 56, and 70 days after planting with a single application of Switch 62.5 WG 42 days after planting through the drip system.
Parameters observed during the study included leaf chlorophyll and leaf nitrogen (with chlorophyll meter) in February and May; fruit sugar (with refractometer) in May; fruit firmness (with penetrometer) in April and May; severity of gray mold (caused by Botrytis cinereae) twice in March and once in May, and other fruit diseases (mucor fruit rot caused by Mucor spp. and Rhizopus fruit rot caused by Rhizopus spp.) once in May 3 and 5 days after harvest (on a scale of 0 to 4 where 0=no infection; 1=1-25%, 2=26-50%, 3=51-75% and 4=76-100% fungal growth); and fruit yield per plant from 11 weekly harvests between 11 March and 14 May 2020. Leaf chlorophyll and nitrogen data for the Abound dip treatment were not collected in February. Data were analyzed using analysis of variance in Statistix software and significant means were separated using the Least Significant Difference test.
Results and Discussion
Leaf chlorophyll content was significantly higher in plants that received drip application of fungicides compared to untreated plants in February while leaf nitrogen content was significantly higher in the same treatments during the May observation. There were no differences in fruit sugar or average fruit firmness among the treatments.
Average gray mold severity from three harvest dates was low and did not statistically differ among the treatments. However, the severity of other diseases was significantly different among various treatments with the lowest rating in Abound transplant dip on both 3 and 5 days after harvest and only 3 days after harvest in plants that received four applications of Rhyme. Unlike the previous year, visible symptoms of the soilborne diseases were not seen during the study period to evaluate the impact of the treatments. However, there were significant differences among treatments for the marketable fruit yield. Highest marketable yield was observed in the treatment that received Rhyme and Switch followed by Velum Prime and Switch and Rhyme alone. The lowest fruit yield was observed in Abound dip treatment. Unmarketable fruit (deformed or diseased) yield was similar among the treatments. Compared to the untreated control, Abound dip resulted in 16% less marketable yield and such a negative impact from transplant dip in fungicides has been seen in other studies (Dara and Peck, 2017 and 2018; Dara, 2020). Marketable fruit yield was 4-28% higher where fungicides were applied to the soil.
Although visible symptoms of soilborne diseases were absent during the study, periodic drip application of the fungicides probably suppressed the fungal inocula and associated stress and might have contributed to increased yields. The direct impact of fungicide treatments on soilborne pathogens was, however, not clear in this study. Considering cost of chemical fumigation or soil disinfestation and the environmental impact of chemical fumigation, treating the soil with fungicides can be an economical option if they are effective. While this study presents some preliminary data, additional studies in non-fumigated fields in the presence of pathogens are necessary to consider soil fungicide treatment as a control option.
Acknowledgments: Thanks to FMC for funding this study and Marjan Heidarian Dehkordi and Tamas Zold for their technical assistance.
References
Dara, S. K. 2020. Improving strawberry yields with biostimulants and nutrient supplements: a 2019-2020 study. UCANR eJournal of Entomology and Biologicals. https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=43631
Dara, S. K. and D. Peck. 2017. Evaluating beneficial microbe-based products for their impact on strawberry plant growth, health, and fruit yield. UCANR eJournal of Entomology and Biologicals. https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=25122
Dara, S. K. and D. Peck. 2018. Evaluation of additive, soil amendment, and biostimulant products in Santa Maria strawberry. CAPCA Adviser, 21 (5): 44-50.
Improving strawberry yields with biostimulants: a 2018-2019 study
Biostimulants are beneficial microorganisms or substances that can be used in crop production to improve plants' immune responses and their ability to perform well under biotic and abiotic stresses. Biostimulants induce plant resistance to stress factors through systemic acquired resistance or induced systemic resistance. When plants are exposed to virulent and avirulent pathogens, non-pathogenic microorganisms, and some chemicals, the systemic acquired resistance mechanism is activated through the salicylic acid pathway triggering the production of pathogenesis-related proteins. On the other hand, when plants are exposed to beneficial microbes, the induced systemic resistance mechanism is activated through the jasmonic acid and ethylene pathways. The jasmonic acid pathway also leads to pathogenesis-related protein production in plants. In other words, when plants are exposed to pathogens, non-pathogens, or other compounds, various defense genes are activated through two major immune responses, helping plants fight the real infection or prepare them for potential infection. Beneficial microbes and non-microbial biostimulants are like vaccines that prepare plants for potential health problems.
Earlier studies in tomato (Dara and Lewis, 2018; Dara, 2019a) and strawberry (Dara and Peck, 2018; Dara, 2019b) demonstrated varying levels of benefits to crop health and yield improvements from a variety of botanical, microbial, or mineral biostimulants and other supplements. Some of the evaluated products resulted in significant yield improvement in both tomatoes and strawberries compared to the grower standard practices. There are several biostimulant products in the market with a variety of active ingredients, and some also have major plant nutrients such as nitrogen, phosphorus, and potassium. Depending on the crop, growing conditions, potential risk of pests and diseases, and other factors, growers can use one or more of these products. A study was conducted to evaluate the impact of various biostimulants on the yield, quality, and shelf life of strawberries.
Methodology
Strawberry cultivar San Andreas was planted late November 2018 and treatments were administered at the time of planting or soon after, depending on the protocol. Each treatment had a 290' long strawberry bed where 10' of the bed at each end was left out as a buffer. Then, six 30' long plots, each representing a replication, were marked within each bed with an 18' buffer between the plots. Since the test products needed to be applied through the drip system, an entire bed was allocated for each treatment, except for the standard program that had one bed on either side of the experimental block, and plots were marked within each bed for data collection. The following treatment regimens were used in the study:
1. Standard Program (SP): Major nutrients were provided in the form of Urea Ammonium Nitrate Solution 32-0-0, Ammonium Polyphosphate Solution, and Potassium Thiosulfate (KTS 0-0-25). Nitrogen, phosphorus, and potassium were applied before planting in November 2018 at 170, 60, and 130 lb/acre, respectively. From 15 January to 9 May 2019, a total of 26 lb of nitrogen, 13 lb of phosphorus, and 26 lb of potassium were applied through 13 periodic applications.
2. SP + Terramera Program: Formulation labeled as Experimental A (cold-pressed neem 70%) was applied at 1.2% vol/vol immediately after planting. Additional applications were made starting from 2 weeks after planting once every two weeks until the end of February (six times), followed by 13 weekly applications from the beginning of March.
3. SP + Locus Low Rate Program: This program contained Rhizolizer soil amendment (Trichoderma harzianum 1X108 CFU/ml and Bacillus amyloliquefaciens 1X109 CFU/ml) at 3 fl oz/acre, humic acid at 13.5 fl oz/acre, and kelp at 6.8 fl oz/acre. The first application was made within 15 days and at 30 days after planting followed by once in February, March, and April 2019.
4. SP + Locus High Rate Program: This program contained Rhizolizer soil amendment (Trichoderma harzianum 1X108 CFU/ml and Bacillus amyloliquefaciens 1X109 CFU/ml) at 6 fl oz/acre, humic acid at 13.5 fl oz/acre, and kelp at 6.8 fl oz/acre. The first application was made within 15 days and at 30 days after planting followed by once in February, March, and April 2019.
5. SP + BioGro Program: Transplants were treated with Premium Plant BB (Beauveria bassiana 1.1%) by spraying 2 fl oz/acre (1.29 ml in 850 ml of water). About 7 weeks after planting, 30 gpa of Plant-X Rhizo-Pro (botanical extracts), 2 gpa of CHB Premium 21 (humic acid blend), 3 gpa of CHB Premium 6 (3% humic acids), and 5 gpa of NUE Flourish 4-12-0 were applied. Starting from mid-February 2019, 15 gpa of Plant-X Rhizo-Pro, 1 gpa of CHB Premium 21, and 2 gpa of CHB Premium 6 were applied four times every 2 weeks until the end of March. Starting from 5 April 2019, 8 weekly applications of 10 gpa of Plant-X Rhizo-Pro, 1 gpa of CHB Premium 21, 2 gpa of Premium 6, and 4 gpa of NUE Flourish 4-12-0 were made until 26 May 2019.
6. SP + Actagro Program: Structure 7-21-0 at 3 gpa and Liquid Humus 0-0-4 with 22% organic acids at 1 gpa were first applied within 1 week of planting and then three more times every 2 weeks until the end of December 2018. Additional monthly applications were made from the end of January to the end of April 2019.
All the fertilizers and treatment materials were applied through the drip system using the Dosatron (Model D14MZ2) equipment. The following parameters were measured during the experimental period from January to May 2019.
Canopy: The size of the plant canopy was determined on 21 January and again on 17 February 2019 by measuring the spread of the canopy across and along the length of the bed from 16 random plants within each plot, and calculating the area.
Initial flowering and fruiting: When flowering initiated, the number of flowers and developing fruits was counted from 16 random plants within each plot on 1 and 16 February 2019.
Fruit yield: Fruit was harvested weekly from every plant within each plot from 3 March to 26 May 2019 on 11 dates and the number and weight of the marketable and unmarketable fruit was determined. Due to a technical error, some of the yield data from an additional date (29 March) were lost and excluded from the analysis.
Fruit firmness: The firmness of two marketable fruit from each of five random plants per plot was measured using a penetrometer on 5 April, and 16 and 26 May 2019.
Fruit sugar content: The sugar content from one marketable fruit from each of 10 plants per plot was measured using a refractometer on 5 April and 26 May 2019.
Leaf chlorophyll content: On 11 March and 31 May 2019, the chlorophyll content of one mature leaf from each of five random plants per plot was measured using a chlorophyll meter.
Postharvest disease: Marketable fruit harvested on 21 and 28 April, and 5 and 26 May 2019 was kept at the room temperature in perforated plastic containers (clamshells) and the growth of gray mold (Botrytis cinerea) or Rhizopus fruit rot fungus (Rhizopus spp.) was measured on a scale of 0 to 4 (where 0=no fungus, 1=1-25%, 2=26-50%, 3=51-75%, and 4=76-100% fungal growth) 3 and 5 days after each harvest.
Data were analyzed using analysis of variance in Statistix software and significant means were separated using the Least Significant Difference means separation test.
Results and Discussion
Statistically significant differences among treatments were seen for the seasonal total number of unmarketable berries (P = 0.0172), the initial flower and fruit numbers on 1 February (P < 0.0014), the leaf chlorophyll content on 31 May (P = 0.0144), and the disease rating 3 days after the 28 April harvest (P = 0.0065).
Treatments did not differ (P > 0.05) in any other measured parameters of the plant, fruit quality, or yield. However, the total seasonal fruit yield was 13 to 31% higher and the total marketable fruit yield was 10 to 36% higher in various treatment programs compared to the standard program. The seasonal total of unmarketable fruit yield was also 4 to 25% higher in treatment programs than the standard program except that there were nearly 12% fewer unmarketable berries in the Actagro program compared to the standard program.
While treatments did not statistically differ for many of the measured parameters, numerical differences in marketable fruit yield could be helpful for some understanding of the potential of these biostimulants. Additional studies with larger treatment plots would be useful for generating additional data.
Acknowledgments
Thanks to Dr. Jenita Thinakaran for the assistance at the start of the study, Hamza Khairi for his technical assistance throughout the study, the field staff at the Shafter Research Station for the crop maintenance, NorCal Nursery for the strawberry transplants, and Actagro, BioGro, Locus, and Terramera for their collaboration and financial support
References
Dara, S. K. 2019a. Improving tomato yield with nutrient materials containing microbial and botanical biostimulants. eJournal of Entomology and Biologicals, 6 June 2019 https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=30448
Dara, S. K. 2019b. Evaluating the efficacy of anti-stress supplements on strawberry yield and quality. eJournal of Entomology and Biologicals, 10 August 2019 https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=31044
Dara, S. K. and D. Peck. 2018. Microbial and bioactive soil amendments for improving strawberry crop growth, health, and fruit yields: a 2017-2018 study eJournal of Entomology and Biologicals, 3 August 2018 https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=27891
Dara, S. K. and E. Lewis. 2018. Impact of nutrient and biostimulant materials on tomato crop health and yield. eJournal of Entomology and Biologicals, 9 January 2019 https://ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=26054