UCR wages $11+ million war against citrus greening disease
Three projects win funding to fight tree-killing bacteria
Managing citrus mealybug – does ant control help?
Sandipa Gautam, Sanjeev Dhungana, and David Haviland
UC Co-operative Extension
Starting 2019/20 season, pest control advisors started noticing citrus mealybug infestations in multiple citrus varieties that continued to increase in acreage in the San Joaquin Valley. Although present in the citrus systems, mealybugs were considered to be a minor pest and kept well under check by natural enemies until recently.
What are mealybugs?
Mealybugs are soft, oval, flat, distinctly segmented insects whose body is covered in white mealy wax. Citrus mealybug, Planococcus citri is the most common species associated with citrus. Females lay ~600 eggs in egg sacs loosely held by white cottony flint. Crawlers are yellowish, and can move or be carried by ants, birds, or the wind to start new infestations. Crawlers feed by sucking sap using straw-like stylets and soon develop a waxy covering (Fig. 1C). Like California red scale, females molt and stay as third instars until mated by a male. Males go through a pupal stage and emerge as adults with wings that fly to seek a mate.
Mealybugs prefer the inside canopy of the tree and can be found under dense leaves, between clusters of fruits, or in other cryptic places where they can be difficult to find, especially when the population is low. As the season progresses and the tree flushes, blooms, and fruit develops, mealybugs move to the parts of the tree where nutrients flow. Ongoing research on seasonal phenology suggest that there are 5-6 generations in the San Joaquin Valley. The first generation starts from overwintering adult/egg populations in late March/early April. The second-generation crawlers/nymphs move to fruit in June/July. The remaining generations each year primarily feed and multiply on the fruit.
Mealybugs produce nutrient rich honeydew that is an attractive food source for ants. Ants have been reported to defend insect colonies from predators and parasitoids. In an early infestation, ant trails can be used as an indicator to locate mealybug infestations or other sap sucking pests. Managing sugar feeding ants in citrus orchards has shown increased biocontrol of sap sucking insects. It is plausible that loss of chlorpyrifos as an ant control tool may have aggravated ants thereby aiding to increase mealybug pressure in recent years.
As mealybugs have become a reoccurring pest in citrus orchards, University of California researchers-initiated studies to work towards developing strategies to manage this pest. Research funded by Citrus Research Board and led by Gautam lab is investigating biology, field ecology, and management of mealybugs. During field visits, our observations have shown that various ant species of were present throughout the growing season attending mealybug colonies.
Does managing ant help suppress mealybug?
UC researchers have documented that managing sugar feeding ants increases biocontrol, thereby reducing the pest pressure of sap sucking insects. When ant densities are were reduced >90%, there was ~90% less mealybug on twigs and complete elimination of mealybug was reported from fruit. However, managing ants has been a challenge since the use of chlorpyrifos was banned. To address this need, UC researchers have worked to develop and test different types of hydrogel beads for delivering insecticide products to sugar-feeding species of ants.
Research led by David Haviland has focused on the experimental use of commercially-available polyacrylamide hydrogel beads for large field-scale applications. In 2023 a large-scale field trial was conducted in a 20-acre grapefruit block in Sanger, California. Two applications of hydrogel beads laced with insecticides were made on Aug 2 and August 30, 2023. Post-treatment evaluations for ant density were done on 24 trees/plot by counting the number of ants that passed through a graft union for 15 secs weekly for 4 weeks after each application. The effects of ant suppression on mealybug densities was evaluated in October 2023 by counting the number of fruits infested with mealybugs (presence/absence) on 30 fruits from the inside canopy. Insecticide treatments had variable results on ant density, with lowest ant density consistently found in plots treated with thiamethoxam. Similarly, plots treated with thiamethoxam had the lowest populations of mealybugs.
There's a lot more to this story:
https://ceventura.ucanr.edu/Com_Ag/Subtropical/?newsletteritem=100493
Update on Novel Ant Control Method in Citrus (MyAgLife in Citrus, Episode 828)
Recently, the Citrus Research Boards, IPM Entomologist, Ivan Milosavljevi?, Ph.D., was a guest on Episode 828 of MyAgLife in Citrus, where he provided an update on on a novel method of ant control in citrus. This method utilizes hydrogel beads filled with trace pesticide amounts.
To listen to the episode, please click below.
Episode 828 | April 30, 2024 | MyAgLife in Citrus – MyAgLife Daily News Report
Thanks to the generosity of the Hrdy family, we are pleased to announce that the following proposals have been selected for funding through The Daniel & Sarah Hrdy Fund for UC Cooperative Extension Research.
Proposals were submitted by individuals or teams. Proposal reviewers ranked the 25 submissions, giving priority to outstanding proposals submitted by early to mid-career level (assistant to associate level) UC ANR academics. Projects will receive three to five years of funding, with up to $25,000 per year. Funds will next be available for proposal submission in 2025.
Brent D. Hales
Associate Vice President, Research and Cooperative Extension
Invasive Fruit Fly Quarantine
What to know as a SJV citrus grower?
Sandipa Gautam
Area Citrus IPM Advisor
UC Statewide IPM Program Operations
Several species of invasive fruit flies that belong to the family Tephritidae are considered serious pests of hundreds of agricultural crops including citrus. These flies lay eggs on or near the fruit surface, and when the maggots hatch, they bore into the fruit, making it unfit for human consumption and causing major losses to fruits and vegetable production. California is experiencing an unusually high number of invasive fruit fly detections in the 2023/24 season and several areas in California are now under a fruit fly quarantine. For many invasive fruit fly species, quarantine is triggered when two or more adult flies are caught in a trap or by a single detection of larvae or pupae indicating a breeding population. Core area is 0.5-mile radius around the detection site and a quarantine area is 4.5-mile radius around each detection.
Counties Currently Impacted by Invasive Fruit Fly Quarantines:
Why is fruit fly quarantine concerning to citrus growers?
Citrus is a known host to all invasive fruit fly species. Fruit fly life cycle begins as eggs laid by adult female on surface or under the fruit rind maggots hatch and bore into the fruit and develop inside the fruit (Figure 5). They drop to the ground and pupate. Many fruit fly species are known to overwinter as prepupae or pupae, but some species like Medfly can overwinter in all life stages inside fruit or as pupae on the ground. Adults emerge in early spring and the life cycle continues. Because eggs and immatures can be present inside the fruit, movement of infested fruit may accidentally transport them to a new area where fruit fly has not been detected.
Invasive fruit flies – what to look for?
Four species of fruit flies are currently regulated in California. The adults may look similar to houseflies but are distinctly different in color and the markings on the body. They are about 5-8 mm in size, much bigger than spotted wing drosophila, another invasive species that has been established in California.
Figure 1. Oriental fruit fly adult with identifying characters (left) and areas in California under quarantine as of January 2024: Contra Costa, Riverside, Sacramento, San Bernardino, and Santa Clara Counties (right).
Figure 2. Mediterranean fruit fly adult with identifying characters (left) and areas in California under quarantine as of January 2024 – Los Angeles County (right).
Figure 3. Tau fruit fly adult with identifying characters (left) and areas in California under quarantine as of January 2024: Los Angeles County (right).
Figure 4. Queensland fruit fly adult with identifying characters (left) and areas in California under quarantine as of January 2024: Ventura County (right).
You can find more information about invasive fruit fly species including interactive quarantine maps, regulatory information and pest profile information below: https://www.cdfa.ca.gov/plant/PDEP/treatment/index.html
Figure 5. Fruit fly larvae are white, legless maggots. They bore into the fruit and feed on pulp. Infested fruit may drop and decay.
What is happening to prevent fruit fly spread?
The California Department of Food and Agriculture (CDFA), in cooperation with the United States Department of Agriculture (USDA) and County Agricultural Commissioners, has initiated local regulatory measures to eradicate and prevent the statewide spread of Queensland fruit fly, Tau fruit fly, Mediterranean fruit fly and Oriental fruit fly. California Citrus Quality Council (CCQC) in coordination with researchers is developing a systems approach that allows for post-harvest treatment of citrus fruit for movement from the core to pack.
What can you do?
If you are a grower inside the quarantine area, follow regulations about harvesting, processing, or storing fruit. Contact your County Ag Commissioner about the latest regulations, or review FAQs here: https://www.cdfa.ca.gov/plant/fruitfly/docs/Invasive_Fruit_Fly_FAQ_Industry_1-18-24.pdf . If your property is under fruit fly quarantine, follow either pre or postharvest treatment protocols from USDA to move fresh fruit
With three new grants totaling more than $11 million, UC Riverside is helping lead the fight against citrus greening or Huanglongbing, a disease threatening citrus industries in the U.S. and worldwide.
The disease is from bacteria transmitted to citrus trees by a tiny flying insect, the Asian citrus psyllid. Infected trees produce no fruit, or fruit that is bitter, small and worthless. Despite intensive research for the past 15 years, there is no known cure for it. It has reduced citrus production in Florida by more than 75%, and it has already been detected in Texas and California.
Because California supplies the country with 80% of its fresh citrus, and because 267,000 acres of Golden State lemons, oranges, grapefruits, and mandarins are at stake if operations are permanently lost, the USDA National Institute of Food and Agriculture is making an emergency investment in citrus disease research with three projects at UCR.
These projects focus on instilling tolerance to the disease with three different approaches: below ground, in the rootstocks, above ground in the shoots and branches, and systemically, with a peptide that would move throughout the tree.
The largest of the projects, at $6.8 million, is being led by Danelle Seymour, assistant professor of genetics in the Botany and Plant Sciences Department at UCR. Their focus is on breeding Huanglongbing or HLB-resistant rootstocks, and the project depends on collaboration with Kim Bowman, a citrus breeder at the USDA Agricultural Research Service in Ft. Pierce, Florida.
“In Florida, nearly every single tree is infected. It's terrible for growers, but wonderful for breeding,” Seymour said. “We can't do this research at large scale in California because the disease isn't as widespread here.”
The classic way to improve resistance or encourage new qualities in crops is through genetics, making crosses between one plant that has a favorable trait, and one that doesn't. “We hope the result is better than the parents,” Seymour said. “When you work in wheat or tomato, you can do these crosses and perform evaluations every year. In citrus, it takes 10 - 15 years to evaluate a new generation of trees.”
Because of the long lag time, the research-ready trees in Florida represent an opportunity for Seymour's team to begin examining new crosses now. The breeder, Kim Bowman, has evaluated over 10,000 trees and unique hybrids, from which a handful will be selected for release to growers.
In addition to evaluating these select few new hybrids for their HLB tolerance, the researchers will be watching the Florida-grown trees' responses to the different environmental conditions in California. “Can they perform well in response to different salinity levels in the soil, different humidity, as well as other pests and pathogens that we have here? We'll find out,” Seymour said.
Chandrika Ramadugu, a project scientist also in UCR's Department of Botany and Plant Sciences, is leading a project to develop HLB-resistant scion varieties that can be grafted to rootstocks. A scion is an above-ground portion of a plant, such as a bud or shoot, that can be used for grafting.
For trees, grafting can be equated to an organ transplant. The scion from one tree is attached to the trunk or rootstock of another with the hope of creating a new plant with combined attributes.
With its grant of $3.28 million, this project will analyze second-generation hybrids that are bred for ten years using Australian lime as a source of disease resistance.
Ramadugu will evaluate 24 novel hybrids in California, Florida, and Texas to assess resistance to HLB. Ideally, in addition to having enhanced disease tolerance, the new plants will also be able to produce good-tasting fruit.
There is little genetic diversity in cultivated citrus. When new pathogens arrive, the genetic uniformity can result in disease epidemics and dire consequences for the crop. In addition to the potential benefits of this project for the fight against HLB, the new hybrids may also help protect citrus from other pests and pathogens.
A third project, granted $1.36 million, will utilize a peptide found in Australian finger limes that is known to impart HLB resistance. Led by Hailing Jin, Microbiology & Plant Pathology professor, the project is developing ways to infuse trees with the peptide.
“The antimicrobial peptide in the finger limes are more efficient at killing bacteria as compared to antibiotics currently used in the field, and much more stable at high temperatures,” Jin said.
Because spray applications are expensive, Jin's project aims to spread the peptide throughout the trees' insides. In collaboration with University of Florida professor Svetlana Folimonova, Jin's team utilizes a natural citrus virus with almost no symptoms to deliver the peptide into the trees.
“You infect the tree with the virus, and it will spread in areas where the bacteria reside,” Jin said. “It would move systemically through the tree, and it would be very cost efficient for growers. No need to buy more insecticides.”
These grants were enabled by the 2018 Agricultural Improvement Act, which authorized the Emergency Citrus Disease Research and Development Trust Fund to fight HLB. With these and other projects, the USDA is bringing together the nation's top scientists to find scientifically sound solutions to the problem in a financially and ecologically sustainable way.