- Author: Ben Faber
Citrus greening disease, also called huanglongbing (HLB), is a bacterial infection of citrus trees that results in small, misshapen and sour fruits that are unsuitable for consumption. The disease ultimately kills the tree.
Because there is no cure, HLB is a major threat to the $10 billion citrus industry in Florida, where it was first detected in 2005,and to the $7 billion industry in California, where it appeared last year.
Researchers from the Boyce Thompson Institute (BTI), the U.S. Department of Agriculture Agricultural Research Service (USDA-ARS) and the University of Washington investigated a seemingly unlikely source of biocontrols for HLB: neuropeptides found in Asian citrus psyllids, the insect that carries the disease-causing bacterium Candidatus Liberibacter asiaticus (CLas), which it spreads while feeding on a tree's leaves and stems.
The research team, led by BTI faculty member Michelle Heck, published its findings on Feb. 10 in the Journal of Proteome Research.
Laura Fleites, a research associate in Heck's group at BTI, focused on neuropeptides because they function as hormones in hemipteran insects – a class that includes psyllids, aphids, whiteflies, shield bugs and other crop-plaguing species – to regulate growth, development and other biological functions.
“If we could develop an insecticide that is specific for Asian citrus psyllids based on one of the insect's own neuropeptides, then we could protect citrus trees from the insect that spreads CLas,” said Heck, a USDA-ARS research molecular biologist and an adjunct associate professor in the School of Integrative Plant Science, in the College of Agriculture and Life Sciences. “Citrus greening disease is devastating our citrus industry, and we need to develop new ways for our citrus growers to control it.”
Fleites and the team characterized the full array of peptides found in the psyllids and identified 122 potential neuropeptides. While promising, the findings offer only potential starting points for combatting HLB, because unmodified insect-derived neuropeptides are not suited for use as insecticides in the citrus grove.
To turn the findings into a usable product, the team is now part of a collaboration aimed at identifying the best psyllid-derived neuropeptide for development. The team will then stabilize the peptide and decide the optimum method for delivering the insecticidal molecule to citrus trees – whether as a spray, by engineering trees to make the peptides themselves, or by some other method.
For the study, Fleites developed new extraction and analysis methods other researchers could use in their investigations of insect peptides.
“Thanks to USDA support, I was able to develop techniques that enable the identification of small, functional insect peptides separately from their larger, inactive precursors,” Fleites said. “Because these techniques aren't specific to psyllids, they may be useful for identifying neuropeptides in other hemipteran insects to protect crop plants.”
Heck said this work shows how USDA grant programs can be used to fund everything from discovery to product development. The neuropeptide discovery was done in a study funded by the National Institute of Food and Agriculture; the translational research is being done under a grant from the Animal and Plant Health Inspection Service.
Funding for this work came from a USDA Specialty Crops Grant and from the USDA-ARS.
Michael J. Haas is a freelance writer for the Boyce Thompson Institute.
https://news.cornell.edu/stories/2020/04/psyllid-peptides-could-fight-citrus-greening-disease
Asian citrus psyllids feed on a citrus tree. The psyllids deposit a bacterium in the sap that causes citrus greening disease, a scourge to the citrus industries in Florida and California, worth a combined $17 billion.
- Author: Ben Faber
New technique has potential
to protect citrus from HLB
Citrus greening, also called Huanglongbing (HLB), is devastating the citrus industry. Florida alone has experienced a 50 to 75 percent reduction in citrus production. There are no resistant varieties of citrus available and limited disease control measures.
Some scientists think it is possible that orange juice could one day become as expensive and rare as caviar. In an effort to prevent this, three plant pathologists at the University of California-Berkeley and United States Department of Agriculture conducted research into ways to boost citrus immunity and protect the valuable fruit against citrus greening.
Because the bacteria that causes citrus greening cannot be grown in a lab, scientists have to find novel ways to conduct experiments. The University of California-Berkeley/USDA team looked at many different strains of the bacteria that cause citrus greening to see if they could identify peptides (a compound of two or more amino acids) that would trigger immune responses.
"This was a long list, so we narrowed it down by selecting small peptides that were a bit different in their peptide sequence, which might imply that the bacterium had made those sequence changes so that they wouldn't be recognized by the plant immune system," explained Jennifer D. Lewis, group leader of the research team. "Then we further narrowed that list to peptides from strains that caused disease in citrus."
Through this research, they showed that two peptides could trigger immune responses in multiple plant species, including citrus. These peptides may play a role in preventing or reducing yield loss from citrus greening.
According to Lewis, "We thought it was particularly interesting that some of the peptides predicted to elicit a response, could actually trigger immune responses in multiple plant species. This suggests that the immune response to these peptides is conserved across species."
/h1>/h1>- Author: Ben Faber
IMMOKALEE, Fla. — University of Florida scientists are working toward establishing a new biological method that may help farmers control the insect that transmits the deadly greening disease into citrus trees. Greening is present in about 95 percent of the citrus trees in Florida, so by using a virus that may kill the insect, growers may be able to reduce the need for pesticides.
Instead of spraying insecticides, scientists hope to harness the natural enemies of the tiny Asian citrus psyllid to manage the invasive pest, said Ozgur Batuman, a plant pathologist at the UF/IFAS Southwest Florida Research and Education Center in Immokalee.
Batuman is leading a UF/IFAS research team investigating viruses that live in the digestive system of the psyllid. They want to see whether any of them can weaken or kill the insect.
The psyllid transmits the bacterium responsible for greening by feeding on a citrus tree's new shoots and leaves.
UF/IFAS scientists are getting promising early results with this research. Their latest research suggests a natural process that would kill the insect or at least prevent greening from being transmitted to citrus trees.
“This invasive pest is now established throughout Florida's commercial groves, so growers use insecticide sprays that are potentially harmful for the environment as one of their primary tactics for fighting citrus greening,” Batuman said. “By reducing psyllid populations in their groves, growers hope to increase the quality of fruits and the productive lives of their trees by minimizing the number of times a tree is exposed to the greening pathogen.”
To arrive at their results, Batuman and his team spent two years collecting psyllids from commercial citrus groves in 22 central and south Florida counties – from Lake and Orange counties in the north to Martin County in the southeast to Collier County in the southwest.
They identified viruses within the psyllids' bodies.
By using a test that amplifies DNA, scientists found five viruses in the guts of the psyllids they collected. They believe they can use those viruses to control the psyllids that live in the very groves from which scientists collected them.
With these experiments, for the first time, scientists have taken big strides toward identifying viruses associated with the Florida psyllids. They also now better understand the presence of those viruses in the psyllids. Asian citrus psyllids first arrived in southeast Florida in 1998.
“Future experiments will investigate how these viruses can be manipulated so that they may alter the pysllid's biology,” said Batuman. “We also need to find out how the viruses affect survival and transmission of the bacterium that causes citrus greening disease, also known as Huanglongbing, or HLB.”
“By understanding how the viruses interact with the psyllid biology, we may be able to understand how to better control the psyllids,” he said.
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By: Brad Buck, 813-757-2224, bradbuck@ufl.edu, 352-875-2641 (cell)
- Author: Ben Faber
Citrus greening disease, or Huanglongbing (HLB), is deadly, incurable, and the most significant threat to the citrus industry. Most HLB research focuses on the tree canopy, but scientists in California studied the impact of HLB on root systems. They recently published the first study to report on the response of two different varieties of citrus to the causal bacterium, 'Candidatus Liberibacter asiaticus' using metabolomics and microbiome technologies.
"Metabolomics is a cutting-edge field of study that provides snapshot information about the metabolism of living things," explains author Emily M. T. Padhi, "while microbiome studies provide valuable information about the microbial communities living in a particular ecological niche - some microbes are beneficial to the host, while others can be harmful."
Padhi and colleagues wanted to see how the root system of two varieties of citrus responded to HLB. They collected roots from healthy and infected Lisbon lemon and Washington Navel orange trees grown in greenhouses at the same time and under the same conditions.
They found that both varieties experienced a reduction in root sugars and amino acids when exposed to HLB. However, they also found differences. While the concentration of malic acid and quinic acid (two metabolites involved in plant defense) increased in the navel roots, they decreased in the lemon roots. They also found that the beneficial bacteria Burkholderia increased substantially in navel plants but not in lemons, which contradicts previous studies.
"Overall, this is the first study to compare two varieties of citrus using a combined metabolomics and microbiome approach and demonstrates that scion influences root microbial community composition and, to a lesser extent, the root metabolome."
There is evidence to suggest that the causal bacterium moves to the root system soon after a plant becomes infected. A key strategy for preserving the health of an infected tree is root system management and research on different responses to HLB may help devise new variety-specific preventative and treatment measures.
MAGE: Images of the bulk root mass and sample leaves from healthy and 'Candidatus Liberibacter asiaticus' lemon and navel plants.
Credit: Emily M. T. Padhi, Nilesh Maharaj, Shin-Yi Lin, Darya O. Mishchuk, Elizabeth Chin, Kris Godfrey, Elizabeth Foster, Marylou Polek, Johan H. J. Leveau, and Carolyn M. Slupsky
- Author: Ben Faber
Citrus greening disease, or Huanglongbing (HLB), is deadly, incurable, and the most significant threat to the citrus industry. Most HLB research focuses on the tree canopy, but scientists in California studied the impact of HLB on root systems. They recently published the first study to report on the response of two different varieties of citrus to the causal bacterium, 'Candidatus Liberibacter asiaticus' using metabolomics and microbiome technologies.
"Metabolomics is a cutting-edge field of study that provides snapshot information about the metabolism of living things," explains author Emily M. T. Padhi, "while microbiome studies provide valuable information about the microbial communities living in a particular ecological niche - some microbes are beneficial to the host, while others can be harmful."
Padhi and colleagues wanted to see how the root system of two varieties of citrus responded to HLB. They collected roots from healthy and infected Lisbon lemon and Washington Navel orange trees grown in greenhouses at the same time and under the same conditions.
They found that both varieties experienced a reduction in root sugars and amino acids when exposed to HLB. However, they also found differences. While the concentration of malic acid and quinic acid (two metabolites involved in plant defense) increased in the navel roots, they decreased in the lemon roots. They also found that the beneficial bacteria Burkholderia increased substantially in navel plants but not in lemons, which contradicts previous studies.
"Overall, this is the first study to compare two varieties of citrus using a combined metabolomics and microbiome approach and demonstrates that scion influences root microbial community composition and, to a lesser extent, the root metabolome."
There is evidence to suggest that the causal bacterium moves to the root system soon after a plant becomes infected. A key strategy for preserving the health of an infected tree is root system management and research on different responses to HLB may help devise new variety-specific preventative and treatment measures.
Emily M. T. Padhi Nilesh Maharaj Shin-Yi Lin Darya O. Mishchuk Elizabeth Chin Kris Godfrey Elizabeth Foster Marylou Polek Johan H. J. Leveau and Carolyn M. Slupsky
https://apsjournals.apsnet.org/doi/10.1094/PHYTO-03-19-0103-R
Images of the bulk root mass and sample leaves from healthy and 'Candidatus Liberibacter asiaticus' lemon and navel plants.