In many ways our pest and disease management of fruit tree crops are exacerbated by our cultural practices. Avocado and citrus offer some very clear demonstrations of how we manage our trees can lead to reduced pesticide use. From the beginning, our selection of rootstock and scion can help lessen pest and disease problems. In both avocado and citrus we have good rootstocks which can handle problems, such as root rot more effectively than seedling rootstocks. So it is imperative that if you know that drainage will be a problem, starting off with the right, healthy rootstock helps. Also scion selection can have a major impact, as well. For example, ‘Lamb' avocado is much less prone to persea mite than is ‘Hass'. This pest can significantly impact a spray program and planting ‘Lamb' could mean virtually no sprays for this pest. Selling the ‘Lamb' fruit is then the challenge There are similar examples in citrus where one variety is more prone to a pest or disease than another.

Irrigation is probably the most important cultural factor in managing tree disease. Over, under and improperly timed irrigations are the conditions necessary for many root diseases. The Phytophthora spp. fungi are looking for distressed root systems brought on by waterlogging and other stressful situations. Other conditions, such as wetted trunks can also bring on some trunk diseases, like gummosis in citrus and crown rot in avocado. Simply preventing irrigation water on the trunks can limit these diseases. Other diseases, such as black streak, stem blight and bacterial canker in avocado are bought on by soil moisture stress.

Nutrients, especially nitrogen management, has been long known to affect levels of insects, such as scale, mealy bug and aphid. Encouraging lush growth helps sustain these insects, so reducing this growth tends to lower their numbers. Managing when canopy growth occurs can affect pest severity. Avocado thrips build their populations in the spring and moves easily from leaf to fruit causing significant scarring. By promoting leaf growth at flowering time with a nitrogen application, keeps the insect on the leaves and reduces fruit scarring. This also promotes growth that replaces leaves that have been damaged by persea mite. Likewise the incidence of citrus leaf miner damage can be reduced if spring pruning is avoided so that a flush of growth does not occur at the same time as the population is building. Timing of pruning is important in lemons to avoid wet periods of rain and fog to reduce the spread of hyphoderma wood rot fungus when its fruiting bodies are active.

Pruning can change pest pressure by changing the humidity in the canopy, introducing light and changing the climate supporting disease and pests. By making spray coverage more thorough, it also makes for a more effective application. Modified skirt pruning can have significant effects on mealy bug and scale control, fuller rose weevil incidence, ant colonization and snail damage. It's important that the trunk be protected as an avenue of movement for snail and ant control to get the best effects of this pruning. Skirt pruning also reduces problems with such weeds as bladder pod and the ladder effect of brown rot in citrus – fungal propagules splashed from the ground onto low-hanging fruit, which in turn is splashed to higher fruit.

Keeping a canopy clean of dust and fire ash also makes for more efficient biological control. Because predators are slowed in their search, they are less efficient. They also spend more time grooming their sensory organs, and this also slows them down. Parasites such as wasps are actually slowed by the physical abrasion to their tarsi. Dust also creates a drier environment, which is more hospitable to our pest mites. Watering picking rows, roads and even the trees themselves can lessen mite populations. Use of cover crops can also reduce dust and potentially provide pollen and nectar for predators and parasites. Of course cover crops create a whole new set of management issues, such as colder winter orchards and snails.

Finally harvest timing to avoid pest and disease is often overlooked. In avocado, fruit is often set in clusters. Greenhouse thrips love the microclimate created, and if in a size-pick the cluster is reduced, greenhouse thrips will often not be a problem. Harvest timing is also important in citrus. Fruit left too long on the tree can often develop septoria fungal spot. Picking in a timely manner reduces the incidence of this disease.

These are just a few examples of how cultural practices at the right time can reduce pest and disease problems.

UF scientists make big stride toward greening-resistant citrus trees

by Brad Buck 

University of Florida scientists achieved a major milestone in their quest to develop a citrus greening-resistant tree by sequencing the genome of a fruit plant that's a close cousin to citrus trees.

You'd need to print 54,000 pages of copy paper to see the complete genome sequence. But within it, scientists believe they've found genes to lay the groundwork to make citrus more tolerant and even resistant to certain diseases, including citrus greening.

UF/IFAS researchers sequenced the genome from trifoliate orange, in collaboration with scientists from the University of California at Berkeley, the U.S. Department of Energy's Joint Genome Institute and UF's Interdisciplinary Center for Biotechnology Research. The new genome will help those who breed new citrus trees that will survive under today's challenging conditions, including invasive pests, viruses and changing climates. Their research provides a powerful new tool to control the deadly consequences of the greening disease, which has severely damaged the state's multibillion dollar-a-year citrus industry.

“Very importantly, trifoliate orange and its hybrids have genes that can confer high tolerance to citrus greening and resistance to the Asian citrus psyllid, the insect that transmits greening to citrus,” said Zhanao Deng, a professor environmental horticulture and a senior author on the new UF/IFAS-led study. “This genome can be used as a reference template to sequence widely used trifoliate orange hybrid rootstock varieties.”

“Most people – even citrus growers – rarely see trifoliate orange. This is because they usually are the rootstock part of the tree, mostly underground,” said Fred Gmitter, a UF/IFAS professor of citrus breeding genetics and a co-author on the study.

Trifoliate oranges or their hybrids are grown at nurseries, and farmers use them as rootstock to grow the citrus that's above ground. Trifoliate orange and its hybrids were used as the rootstock for more than three million citrus trees in Florida alone in 2018-2019, UF/IFAS researchers say.

Trifoliate orange and its hybrid rootstocks accounted for 82% of the top 20 rootstocks used in the 2018-2019 citrus propagation cycle in Florida.

“Our trifoliate orange genome will allow scientists to develop new tools that can more speedily transfer beneficial genes into sweet oranges, grapefruit and breeding of new scion cultivars, which grow above the ground,” Deng said.

“Releasing the first trifoliate orange genome can be valuable for our citrus gene-editing efforts,” Gmitter said. Scientists are using gene editing to produce canker-resistant and greening-tolerant citrus.

“Because of our high-quality genome, re-sequencing of trifoliate orange hybrid rootstock varieties will be much easier, much quicker and much more cost-efficient,” said Deng. “Re-sequencing will enable development of new breeding tools, such as DNA marker-based selection, genomic selection of new rootstock varieties with resistance and tolerance to citrus greening, citrus tristeza virus and citrus nematodes. The new varieties might give higher yield and fruit quality.”

Citrus breeders want to introduce desirable genes from trifoliate orange into sweet orange, grapefruit and other varieties. It took decades to produce the first citrus scion variety (‘Sun Dragon') from crossing trifoliate orange and transferring some of its genes across multiple generations into sweet orange. With this new information from genome sequencing, that timeline can be dramatically reduced.

This project was funded by two grants from the Citrus Research and Development Foundation (CRDF) and a grant from the USDA/NIFA Citrus Disease Research and Extension (CDRE) program.

To see a video about the research and its implications, click here.

FredGmitter at it.  Photo credit: Brad Buck, UF/IFAS.

WASHINGTON, D.C. (September 14, 2020) –Going directly to those hurt most by the devastating citrus greening disease, researchers are asking organic citrus growers and other organic stakeholders to help them develop a holistic research program in their fight against the ruinous disease.
 
Citrus greening disease has been decimating domestic citrus in the United States over the past decade, and organic growers have been hit especially hard with few effective tools to control the disease. A research team led by the University of Florida is collaborating with The Organic Center, University of California, Riverside, a number of organic citrus growers and industry members to conduct a national review of how citrus greening disease is adversely impacting organic growers and other industry members.

Researchers are asking organic growers to fill out a survey based on their knowledge and experience about this devastating disease on their citrus crops. Here is the survey link. Information gathered will be used to develop a large-scale holistic research project proposal targeted toward protecting organic citrus growers from citrus greening, slowing its spread, and reducing damage to currently infected groves. 

For more information, please contact
Petr Kosina, with UC IPM, at pkosina@ucanr.edu