Posts Tagged: Metarhizium brunneum
First report of entomopathogenic fungi, Beauveria bassiana, Isaria fumosorosea, and Metarhizium brunneum promoting the growth and health of cabbage plants growing under water stress
Entomopathogenic fungi such as Beauveria bassiana (commercial formulations, BotaniGard and Mycotrol), Isaria fumosorosea (NoFly and Pfr-97), and Metarhizium brunneum (Met52) are primarily used for controlling arthropod pests. Research in the recent years evaluated their endophytic (colonizing plant tissues) and mycorrhiza-like (associated with roots) relationship with plants and potential benefits in improving plant growth and health. Studies conducted in California showed that B. bassiana endophytically colonized strawberry plants and persisted for up to 9 weeks in various plant tissues (Dara and Dara, 2015a); promoted strawberry plant growth (Dara, 2013); and negatively impacted green peach aphids through endophytic action (Dara, 2016). Soil application of M. brunneum appeared to have a positive impact on strawberry plants in withstanding twospotted spider mite infestations (Dara and Dara, 2015b). Similarly, M. anisopliae reduced the salt stress in soybean (Khan et al., 2012) and M. robertsii enhanced root growth and nutrient absorption in switch grass and haricot beans (Behie et al., 2012; Sasan and Bidochka, 2012). In another study, nitrogen obtained from an insect host through infection (entomopathogenic relationship) was transferred by B. bassiana and Metarrhizum spp. to a plant through an endophytic or mycorrhiza-like relationship.
Several beneficial microbe-based products are commercially available to promote plant growth under normal or stressful conditions and to boost plant defenses against pests and diseases. However, several mycorrhizae do not form a symbiotic relationship with several cruciferous hosts and mycorrhizae-based products are typically not used in cole crops. If entomopathogenic fungi, which have a great promise for pest management in IPM programs, could also promote plant growth and health through an endophytic or mycorrhiza-like relationship, they will maximize their potential for multipurpose use in crop protection and production and potentially reduce the cost of applying multiple products for multiple purposes.
A study was conducted in 2014 to evaluate the impact of B. bassiana, I. fumosorosea, and M. brunneum on potted cabbage plants growing in artificial light with reduced water.
Methodology
About 3-week old cabbage (var. Supreme Vantage) transplants (obtained from Plantel Nurseries, Santa Maria, CA) were planted in Miracle-Gro® Moisture Control Potting Mix (NPKFe 0.21-0.07-0.14-0.10) in 650 ml containers. Treatments included BotaniGard ES (1 ml), Met 52 EC (1 ml), NoFly WP (2.5 mg), SumaGrow (2.3 ml), CropSignal (1 ml), Mykos Liquid (0.03 ml), and H2H (10 ml) in 100 ml of water which were added to each container in respective treatments. Miracle-Gro alone was used as the control. Each treatment had 10 plants which were grown under artificial lighting (75 W plant light in each corner). To each container, 50 ml of water was added again on 42, 50, 64, and 81 days after planting. Temperatures during the study were 56o (minimum), 71o (average), and 88o F (maximum).
Treatments used in the study
Data were collected as follows:
- Plant health rating was recorded at 40 and 70 days after planting on a scale of 0 to 5 where 0=dead, 1=weak, 2=moderate-low, 3=moderate-high, 4=good, and 5=very good.
- Plant survival was recorded at 40, 70, and 90 days after planting.
- Shoot and root length were recorded at 90 days after planting by unearthing each plant from the containers.
- Shoot-to-root ratio was calculated.
- Plants from each treatment were placed in paper bags and dried in an oven at 98oF for 8 days. Dry weight (biomass) of the plants was measured before sending them to an analytical lab for nutrient analysis.
Data were subjected to analysis of variance and significant means were separated using Least Significant Difference test. Since some treatments had fewer plants by the end of the study, biomass measurement and nutrient analysis were done together for all the remaining plants and those two parameters were not subjected to statistical analysis.
Results
Plant survival: Beauveria bassiana was the only treatment where all the plants survived for 90 days of the observation period. There was a 10 to 80% mortality in other treatments during the observation period. Highest plant mortality was seen in SumaGrow and H2H treatments (P = 0.001 at 40 days after planting and
Plant health: Plants treated with B. bassiana were significantly and uniformly healthier (P < 0.00001) than the rest of the treatments on both observation dates with a ‘very good' rating. Health of the plants growing in Miracle-Gro with no supplements also had a ‘good' rating and was better than the health of plants in most of the remaining treatments. Plants treated with SumaGrow and H2H had poor health with a ‘weak' rating.
Shoot and root length: Plants treated with B. bassiana and M. brunneum had significantly (P < 0.00001) longer shoots than other treatments. Miracle-Gro-treated plants were shorter than those treated with these two entomopathogenic fungi, but longer than those in the remaining treatments. When root growth was compared, plants growing in Miracle-Gro alone and along with Crop Signal had significantly (P < 0.00001) longer roots than the rest.
Shoot-to-root ratio: Beauveria bassiana and M. brunneum treatments contributed to a significantly (P < 0.00001) higher ratio than the rest of the treatments.
Biomass and nutrient absorption: Plants treated with B. bassiana had relatively higher biomass. When the plant weight as a result of accumulated nutrients was calculated by dividing the weight with respective nutrient content, B. bassiana appeared to have relatively higher output for nitrogen, phosphorus, and potassium based on numerical values. Such an effect for iron was seen in all, except H2H, treatments compared to Miracle-Gro alone. However, these values are only indicative as they were not subjected to statistical analysis.
This is the first report of the direct impact of entomopathogenic fungi on cabbage plant growth. Beauveria bassiana and to some extent M. brunneum had a positive impact on plant growth and health even under reduced water conditions. If they could be used to promote plant growth, improve water and nutrient absorption, withstand saline or drought conditions, increase yields in addition to their typical use as biopesticides, then they can play a critical role as holistic tools in sustainable agriculture.
Acknowledgements: Thanks to Plantel Nurseries Inc. for donating cabbage transplants, and Advanced Soil Technologies, Bioworks Inc, California Safe Soil, Novozymes Biologicals, Reforestation Technologies International, and SumaGrow USA for various treatment materials used in this study.
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References:
Behie, S.W., P.M. Zelisko, and M.J. Bidochka. 2012. Endophytic insect-parasitic fungi translocate nitrogen directly from insects to plants. Science 336: 1576-1577.
Dara, S. K. 2013. Entomopathogenic fungus, Beauveria bassiana promotes strawberry plant growth and health. UCCE eNewsletter Strawberries and Vegetables, 30 September, 2013. (//ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=11624)
Dara, S. K. and S. R. Dara. 2015a. Entomopathogenic fungus, Beauveria bassiana endophytically colonizes strawberry plants. UCCE eNewsletter Strawberries and Vegetables, 17 February, 2015. (//ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=16811)
Dara, S. K. and S. R. Dara. 2015b. Soil application of the entomopathogenic fungus, Metarhizium brunneum protects strawberry plants from spider mite damage. UCCE eNewsletter Strawberries and Vegetables, 18 February, 2015. (//ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=16821)
Dara, S. K. 2016. Endophytic Beauveria bassiana negatively impacts green peach aphids on strawberries. UCCE eNewsletter Strawberries and Vegetables, 2 August, 2016. (//ucanr.edu/blogs/blogcore/postdetail.cfm?postnum=21711)
Sasan, R.K. and M.J. Bidochka. 2012. The insect-pathogenic fungus Metarhizium robertsii (Clavicipitaceae) is also an endophyte that stimulates plant root development. Amer. J. Bot. 99:101-107.
/h4>Soil application of the entomopathogenic fungus Metarhizium brunneum protects strawberry plants from spider mite damage
Entomopathogenic fungus Beauveria bassiana is known to endophytically colonize various plants and provide protection against arthropod pests. Information of such endophytic interaction of another entomopathogenic fungus Metarhizium brunneum (=M. anisopliae) is limited.
A greenhouse study was conducted in 2010 to evaluate the endophytic potential of B. bassiana (commercial isolate GHA and a California isolate SfBb1) and M. brunneum (commercial isolate F52 and a California isolate GmMa1). Strawberry plants were grown in pots and fungal inocula were applied to the potting medium, vermiculite. When roots and aerial parts were periodically sampled, surface sterilized, and plated on selective media, B. bassiana grew from roots, petioles, pedicels, leaf lamina, sepals, and calyxes whereas M. brunneum was never detected from those tissues. It was initially thought that M. brunneum did not colonize strawberry plants.
However, there was an accidental infestation of twospotted spider mite, Tetranychus urticae on strawberry plants meant for another repetition of the endophyte study with M. brunneum isolates. Among those plants, 32 were treated with M. brunneum isolates and 20 were untreated control plants. Treatments were administered by applying 100 ml of conidial suspension at 1X10^10 conidia/ml concentration around the base of each potted plant. Each isolate had 16 strawberry plants. Mite counts were not taken as the plants were initially intended for endophyte evaluation and leaves could not be destructively sampled. But the proportion of plants damaged by mite infestations were recorded 10 and 14 days after fungal inoculation.
Plants treated with M. brunneum isolates appeared to withstand spider mite infestations better than untreated controls. Since M. brunneum could not be detected in the plant tissue in the previous attempt, it was not clear at that time how the fungus helped strawberry plants to withstand mite damage.
A recent study using scanning electronic microcopy showed that M. brunneum endophytically colonized cowpea plants. It is possible that M. brunneum colonized strawberry plants, but could not be detected using selective medium technique. Another study demonstrated that B. bassiana and M. brunneum promoted the growth of cabbage plants and improved the biomass. In the current study, M. brunneum probably improved the moisture absorption in strawberry plants through mycorrhizal interaction and helped withstand the spider mite infestations which are usually worse in plants under water stress. Fungal toxins in strawberry plants might have also impacted spider mites in a manner similar to the effect of endophytic B. bassiana on green peach aphid, Myzus persicae, in a different study. Observations from the current study indicate the potential of M. brunneum as an endophyte in protecting plants from arthropod damage. Additional studies are required to further investigate this interaction.
Acknowledgment: Thanks to Dale Spurgeon, USDA-ARS for providing laboratory and greenhouse resources for this study.
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References
Dara, S. K. and S. R. Dara. 2015. Entomopathogenic fungus Beauveria bassiana endophytically colonizes strawberry plants. UCANR eNewsletter Strawberries and Vegetables, February 17, 2015.
Dara, S. K., S. S. Dara, and S. S. Dara. 2014. Entomopathogenic fungi as plant growth enhancers. 47th Annual Meeting of the Society for Invertebrate Pathology and International Congress on Invertebrate Pathology and Microbial Control, August 3-7, Mainz, Germany, pp. 103-104.
Golo, P. S., W. Arruda, F. R. S. Paixão, F. M. Alves, E.K.K. Fernandes, D. W. Roberts, and V.R.E.P. Bittencourt. 2014. Interactions between cowpea plants vs. Metarhizium spp. entomopathogenic fungi. 47th Annual Meeting of the Society for Invertebrate Pathology and International Congress on Invertebrate Pathology and Microbial Control, August 3-7, Mainz, Germany, pp. 104.
Vega, F. E., F. Posada, M. C. Aime, M. Pava-Ripoll, F. Infante, and S. A. Rehner. 2008. Entomopathogenic fungal endophytes. Biol. Con. 46:72-82.