A workshop on anaerobic soil disinfestation (ASD) will take place at the Watsonville UC Cooperative Extension office on August 17.

Anaerobic Soil Disinfestation (ASD) was developed in the Netherlands and Japan as a biological alternative to methyl bromide fumigation. Funded by USDA and the California Strawberry Commission, our research team has been working on optimizing ASD for California strawberries since 2007. Recent data showed that the ASD technique produced comparable marketable fruit yield with a fumigant (Pic-Clor 60) treatment. Although ASD technology is still a work-in-progress, many growers have expressed interests in learning about ASD. This workshop is designed to cover the basics and key aspects for successful ASD implementation.

 When: 8/17/12 (F) 9-11am

Where: Auditorium at Santa Cruz County Cooperative Extension 1432 Freedom Boulevard, Watsonville, CA 95076-2796
Phone: (831) 763-8040

Agenda:

9-9:30am:

Anaerobic Soil Disinfestation (ASD): Basics and Research Update

               Carol Shennan, Professor, Dept. of Environmental Studies. University of California, Santa Cruz.

9:30-10am:

Key Practices for Successful ASD Implementation

               Joji Muramoto, Associate Researcher, Dept. of Environmental Studies. University of California, Santa Cruz.

10-10:20am:

Carbon Source Supplies and Consulting Services for ASD

               Kurt Jacobson, Farm Fuel. Inc.

10:20-11am:

Questions and Answers

11am:

Adjourn

For more information, call Joji Muramoto at 831-247-3804.

Introduction: The following is a description of a second, more thorough evaluation of the situation described previously in this space of yellow strawberry plants in a field in Castroville.  This issue of yellow strawberry plants popping up in certain locations in the Castroville – Salinas production area has confounded us for years, but I believe the work described below allows us to make a strong argument as to the cause is of this malady at least in this case.

Materials and Methods:  A total of four samples (two from an area of severe yellowing, and two from an area of apparently healthy green plants) were taken.  Following the output example posted below (Figure 2), each sample consists of 10 zones of a bed, and each zone is tested for 14 parameters.  At each soil sampling site, a representative plant sample was uprooted and taken away for analysis of tissue mineral concentration.

Results: The data in the Tables 1 and 2 below represent an average of the two samples taken each for yellow and green plants.  In order to better interpret the data, several zones have been grouped together.  Zones 9 and 10 represent the surface of the bed, zones 1, 2 and 3 represent the soil straight underneath the drip tape, zone 8 the plant zone, zone 7 the root zone and zones 6, 5 and 4 being underneath the root zone 7.

Table 1: Evaluation of Zones 1- 6 of Bed in Yellow and Healthy Areas

Table 2: Evaluation of Zones 7-10 of Bed in Yellow and Healthy Areas

Table 3: Comparison of Mineral Concentrations of Leaf Tissue for Green and Yellow Plants

I’ve also taken a look at the irrigation water.  As is common in northern Monterey county, the farm gets its water as a mixture of recycled water blended with well or river water.  A full report for an example of the blended irrigation water used on this farm is available from the Monterey Regional Water Pollution Control Agency at:

http://www.mrwpca.org/recycling/chem2012_blended.php

In the sample taken of the blended recycled and river water mix, conductivity (EC) was 1.3 dS/m, sodium 118 ppm, chloride 160 ppm and adjusted SAR of 3.4 (sodium adsorption ratio, an index of sodium hazard adjusted for the amount of calcium in the irrigation water). 

Discussion:  The pH of the soil in the beds in all zones is quite high, which is not surprising because of the high percentage of carbonates (lime) throughout.  One can also see accumulations of nitrates, phosphates and potassium are substantially higher in the areas of the yellow plants, which quite likely due to their declining ability to take up the nutrients being continually applied as fertilizer.   It is worth noting that nitrates in the high concentrations found in our soil tests can be toxic to plants, thus accelerating the decline of plants.

Nitrites, generated from ammonium in anaerobic conditions, are zero, indicating adequate aeration in the bed.

In terms of the irrigation water, referring to the water quality guidelines for crops developed by UC Cooperative Extension, we find that the water used on this farm can be used with some restriction to irrigate crops moderately susceptible to salinity such as strawberry.  In other words, this irrigation water isn’t great but is OK.

It seems that the real culprits in this field are the accumulated amounts of chloride and sodium.  Generally speaking, crops in our area perform best when the soil sodium levels are less than 250 ppm and soil chloride levels are less than 100 ppm. 

Across all the soil samples the average amounts of sodium and chloride are above 250 ppm and 100 ppm respectively.   High as they are, the concentrations of both ions do not vary greatly in the plant and root zones and the zones around them in either areas of green or yellow plants. There are, however, substantial differences in the concentrations of these ions at the crust (zones 9 and 10) between green and yellow plant areas. For example, on average soil from areas of yellow plants there is nearly a twofold accumulation of sodium in the crust of soil taken as well as a substantially higher amount of chloride over the soil sampled around green, healthy plants.  This tells us that while these large amounts of sodium and chloride (also known as salts) are accumulating away from the plant via evaporation, they are nevertheless passing through more sensitive plant zones to get there.  It is not difficult then to hypothesize that during this transition the yellow plants are picking up these salts obtaining the fourfold accumulation of sodium and 40% increase in chlorides that we observe in the leaf tissue of yellow plants over that of green.

Furthermore, it is notable that the amount of lime (CaCO3) in all the soil samples is high.   This indicates that a lot of calcium coming into the soil from irrigation water is precipitating out and not as capable of sufficiently limiting the amount of exchangeable sodium.   In turn the sodium hazard is high.  This may also explain to some extent why areas quite close to one another respond differently, since in some areas (perhaps even quite close together) more calcium precipitates out than others.

My conclusion drawn from all this work in this particular instance of severe plant yellowing of strawberry plants is that the uptake of sodium and chloride by these affected plants is very high and they are being poisoned by salt.

Thank you to Frank Shields and Soil Control Lab for their generous assistance in this work.  Thanks to other colleagues for their valuable insight and input which assisted me in the development of my conclusion.

This project was supported in part with funds provided by the California Strawberry Commission.

The following is a description and evaluation of tip dieback in Arapaho blackberry in a field outside of Watsonville .

As the reader can see from the pictures below, the issue is a rather abrupt manifestation of wilt, followed by a blackening of the tip.  Cutting back the outer layer of the cane reveals (Photo 2 below) that the necrosis is advancing in the interior of the cane, but as with the tip blackening itself, seems to be restricted to the top 4 or 5 inches.

This problem of tip dieback was reported some weeks ago on a few tips of the Arapaho planting and none of the other varieties in the same field.  As of last week this dieback has advanced through most of the planting and most of the tips are now affected and again restricted only to Arapaho. 

There are no signs, for example oozing typical of bacteria or fruiting bodies or mycelium associated with a fungus, of disease.  Seeing that the tip dieback is similar to the blackening of young leaf tissue caused by insufficiency of calcium ( for example black heart in celery) we tested with a two replicate sample the nutritional status of cane tips just starting to wilt and compared them to others still apparently healthy.  The output of this evaluation is below.  Also please note that substantial portions of cane were included in the sample, which would be expected to have different - probably lower - levels of minerals than the normally tested leaf tissue. 

Table 1: Comparison of Mineral Concentrations of Healthy and Tip Burned Tissue on Arapahoe Blackberry.

Concentrations of nitrogen, phosphorous, potassium, sulfur, copper and zinc are higher in the healthy tips, while concentrations of manganese, calcium, sodium and chloride are higher in the wilted tips.  While the levels of chloride are nearly double in the cane which has died back, these levels of chloride are still not consistent with what we know to be damaging to plant tissue, usually ranging about 3000 or 4000 parts per million.

Considering that if the problem is not a disease, nor nutritional, what is it then?  The answer comes to us from people with experience with the variety and the problem.  We go to John Clark from the University of Arkansas, who has noted previously this very tip dieback on Arapaho blackberry in cooler climates (for which the summer Central Coast obviously qualifies) to the extent that he cautions growers in cooler regions about planting it. 

In conclusion we can be fairly certain that the tip burning we are currently observing here in Arapaho blackberry is physiological and caused by an interaction of the variety and our climate.

The 2012 Monterey Bay Academy Field Day is planned for this coming August 16 with registration starting at 8:30 am.  Agenda items are are a general discussion of growing strawberries in substrate, fruit and foliar pathogens, lygus bug management, anaerobic soil disinfestation, use of steam and mustard meal as soil sterilants and the use of Zeus herbicide.

Please refer to the agenda attached below for further information:

http://cesantacruz.ucdavis.edu/files/147807.pdf