Posts Tagged: USDA

UC Davis awarded grant to advance strawberry breeding, genetic tools

The $6.2 million grant centers on protecting crops in the future

The federal government is awarding $6.2 million to University of California, Davis, to study how to use breeding and genetic information to protect strawberry crops from future diseases and pests.

The four-year grant from the National Institute of Food and Agriculture (NIFA) centers on addressing expanding and emerging threats to strawberries, a popular fruit packed with Vitamin C and key to the diets of many Americans.

Enhanced plant breeding, gene editing and other technologies will be key to ensuring strawberry crops are sustainable in the face of climate change and possible restrictions on chemical use, said Steve Knapp, director of the Strawberry Breeding Center and a distinguished professor in the Department of Plant Sciences.

“We need to have the technology so that we can deal with the challenges strawberries face around the world,” Knapp said. “Can we use genetic knowledge to change the DNA in a specific way to get the resistance we need?”

USDA funding

The grant award was one of 25 announced Oct. 5 by NIFA – an agency of the U.S. Department of Agriculture – as part of the Specialty Crop Research Initiative program, which addresses “key challenges of national, regional and multistate importance in sustaining all components of food and agriculture…,” the agency said.

The strawberry industry has lagged behind crops like tomato and wheat when it comes to genetic and technical innovation, Knapp said, and the grant signifies that “now they want the foot on the accelerator.”

A key priority is identifying whether changing DNA molecules can improve disease resistance and what technologies would be needed. Ensuring some genes are expressed while others are suppressed would be part of the analysis.

“We're trying to build in natural resistance to pathogens through the genes that already exist but could be modified with this knowledge,” Knapp said. “If we were able to edit a gene that improves disease resistance, people would want us to use that in breeding.”

The intent is to produce disease-resistant cultivars and identify better ways to diagnose, prevent and manage disease. The research project will also include an economic forecast evaluating the consequences of production changes and communicating with farmers about the laboratory advances, according to the grant proposal.

Gitta Coaker from plant pathology and Mitchell Feldmann, Marta Bjornson and Juan Debernardi from plant sciences are participating in the research, as are scientists from California Polytechnic State University, UC Agriculture and Natural Resources, UC Berkeley, University of Florida and USDA's Agricultural Research Service.

UC Davis to lead $15 million research into climate-change resistant wheat

The project will also train plant breeders for the future

Wheat products account for roughly 20% of what people eat every day around the globe. As climate changes, wheat crops must adapt to new weather patterns to keep up with demand.

The University of California, Davis, is leading a five-year, $15 million research project to accelerate wheat breeding to meet those new climate realities, as well as to train a new generation of plant breeders.

“Everything is less stable,” said Jorge Dubcovsky, a plant sciences distinguished professor who is leading the grant research. “Everything is changing so you need to be fast. You need to be able to adapt fast.”

The grant from the U.S. Department of Agriculture's National Institute of Food and Agriculture will create a coordinated consortium of 41 wheat breeders and researchers from 22 institutions in 20 states. Researchers from Mexico and the United Kingdom are also participating.

Breeding needs to speed up

“Breeding crops for the future will require new traits, breeding platforms built for quick transfer of traits to elite cultivars, coordination of breeding efforts in public and private domains, and training for current and future plant breeders and researchers,” NIFA said in an announcement about this grant and others related to breeding.

The program involves on-the-ground research, identifying molecular markers and data analysis from multiple institutions to determine genes that will help wheat crops mitigate the effects of climate change. Plant breeding will follow to prove out those findings.

Wheat is unlike other crops in that 60% of the plant varieties — generating about $4 billion in annual production — are developed by public breeding programs rather than private corporations. In many states, wheat growers tax themselves to support basic breeding efforts at public institutions like UC Davis.

Increased coordinated research

The NIFA grant money will lead to more coordinated, sophisticated research. “This grant allows us to do breeding at a level that a good, modern company would do,” Dubcovsky said. “This grant is essential to maintain modern and effective public breeding programs in the U.S.”

The consortium will bring together data and research from across institutions, allowing for more expansive analysis while reducing redundancies. “We can take advantage of the data from everybody,” he said. “By doing that we don't need to duplicate efforts.”

A team in Texas will analyze plant images taken from drones at each institution to extract information about plant growth, water use, nitrogen levels and other data. “Using technology, we can see beyond our human capabilities,” Dubcovsky said. “You can extract a huge amount of information from every plant variety.”

The data from those images will allow researchers to document the plants throughout the life cycle and determine which plants fare better under certain conditions. Genotyping will help researchers obtain information about the plant genome. The combination of these two types of data could speed up breeding cycles, helping wheat crops adapt to a changing environment.

“If we can breed fast, we can adapt to change,” Dubcovsky said. “We are trying to make sustainable improvements in time.”

Training the next generation

The project will also train a cohort of 20 plant Ph.D. students in active breeding programs where they will participate in fieldwork, collect data from drones and DNA samples, and learn to integrate that information to accelerate wheat breeding. The students will participate in online and face-to-face workshops, as well as educational events and national scientific conferences.

Colorado State University, Cornell University, Kansas State University, Michigan State University, Montana State University, Oklahoma State University, Purdue University, South Dakota State University, Texas A&M University, University of Idaho, University of Illinois, University of Minnesota, University of Nebraska, University of Wisconsin, Utah State University, Virginia Tech, Washington State University, and U.S. Department of Agriculture Agricultural Research Service branches in North Dakota, Washington, Kansas and North Carolina are also participating in the consortium.

Citrus threat target of $7 million multistate research project

UC ANR part of team led by Texas A&M AgriLife combating huanglongbing disease

Citrus greening, or huanglongbing disease (HLB), is the most devastating disease for orange and grapefruit trees in the U.S. Prevention and treatment methods have proven elusive, and a definitive cure does not exist.

Since HLB was detected in Florida in 2005, Florida's citrus production has fallen by 80%. Although there have been no HLB positive trees detected in commercial groves in California, more than 2,700 HLB positive trees have been detected on residential properties in the greater Los Angeles region.

“It is likely only a matter of time when the disease will spread to commercial fields, so our strategy in California is to try to eradicate the insect vector of the disease, Asian citrus psyllid,” said Greg Douhan, University of California Cooperative Extension citrus advisor for Tulare, Fresno and Madera counties.

Now, a public-private collaborative effort across Texas, California, Florida and Indiana will draw on prior successes in research and innovation to advance new, environmentally friendly and commercially viable control strategies for huanglongbing.

Led by scientists from Texas A&M AgriLife Research, the team includes three UC Agriculture and Natural Resources experts: Douhan; Sonia Rios, UCCE subtropical horticulture advisor for Riverside and San Diego counties; and Ben Faber, UCCE advisor for Ventura, Santa Barbara and San Luis Obispo counties.

$7 million USDA project

The $7 million, four-year AgriLife Research project is part of an $11 million suite of grants from the U.S. Department of Agriculture National Institute of Food and Agriculture, NIFA, to combat HLB. The coordinated agricultural project is also a NIFA Center of Excellence.

“Through multistate, interdisciplinary collaborations among universities, regulatory affairs consultants, state and federal agencies, and the citrus industry, we will pursue advanced testing and commercialization of promising therapies and extend outcomes to stakeholders,” said lead investigator Kranthi Mandadi, an AgriLife Research scientist at Weslaco and associate professor in the Department of Plant Pathology and Microbiology at the Texas A&M College of Agriculture and Life Sciences.

The UC ANR members of this collaboration will be responsible for sharing findings from the research with local citrus growers across Southern California, the desert region, the coastal region and the San Joaquin Valley.

“In addition to the ground-breaking research that will be taking place, this project will also help us continue to generate awareness and outreach and share the advancements taking place in the research that is currently being done to help protect California's citrus industry,” said Rios, the project's lead principal investigator in California.

Other institutions on the team include Texas A&M University-Kingsville Citrus Center, University of Florida, Southern Gardens Citrus, Purdue University and USDA Agricultural Research Service.  

“This collaboration is an inspiring example of how research, industry, extension and outreach can create solutions that benefit everyone,” said Patrick J. Stover, vice chancellor of Texas A&M AgriLife, dean of the Texas A&M College of Agriculture and Life Sciences and director of Texas A&M AgriLife Research.  

HLB solutions must overcome known challenges

An effective HLB treatment must avoid numerous pitfalls, Mandadi explained.

One major problem is getting a treatment to the infected inner parts of the tree. The disease-causing bacteria only infect a network of cells called the phloem, which distributes nutrients throughout a tree. Starved of nutrients, infected trees bear low-quality fruits and have shortened lifespans.

Treatments must reach the phloem to kill the bacteria. So, spraying treatments on leaves has little chance of success because citrus leaves' waxy coating usually prevents the treatments from penetrating.

Second, while the bacteria thrive in phloem, they do not grow in a petri dish. Until recently, scientists wishing to test treatments could only do so in living trees, in a slow and laborious process.

Third, orange and grapefruit trees are quite susceptible to the disease-causing bacteria and do not build immunity on their own. Strict quarantines are in place. Treatments must be tested in groves that are already infected.

Two types of potential HLB therapies will be tested using novel technologies

The teams will be working to advance two main types of treatment, employing technologies they've developed in the past to overcome the problems mentioned above.

First, a few years ago, Mandadi and his colleagues discovered a way to propagate the HLB-causing bacteria in the lab. This method involves growing the bacteria in tiny, root-like structures developed from infected trees. The team will use this so-called “hairy roots” method to screen treatments much faster than would be possible in citrus trees.

In these hairy roots, the team will test short chains of amino acids – peptides – that make spinach naturally resistant to HLB. After initial testing, the most promising spinach peptides will undergo testing in field trees. To get these peptides to the phloem of a tree, their gene sequences will be engineered into a special, benign citrus tristeza virus vector developed at the University of Florida. The citrus tristeza virus naturally resides in the phloem and can deliver the peptides where they can be effective.

“Even though a particular peptide may have efficacy in the lab, we won't know if it will be expressed in sufficient levels in a tree and for enough time to kill the bacteria,” Mandadi said. “Viruses are smart, and sometimes they throw the peptide out. Field trials are crucial.”

The second type of treatment to undergo testing is synthetic or naturally occurring small molecules that may kill HLB-causing bacteria. Again, Mandadi's team will screen the molecules in hairy roots. A multistate team will further test the efficacy of the most promising molecules by injecting them into trunks of infected trees in the field.

A feasible HLB treatment is effective and profitable

Another hurdle to overcome is ensuring that growers and consumers accept the products the team develops.

“We have to convince producers that the use of therapies is profitable and consumers that the fruit from treated trees would be safe to eat,” Mandadi said.

Therefore, a multistate economics and marketing team will conduct studies to determine the extent of economic benefits to citrus growers. In addition, a multistate extension and outreach team will use diverse outlets to disseminate project information to stakeholders. This team will also survey growers to gauge how likely they are to try the treatments.

“The research team will be informed by those surveys,” Mandadi said. “We will also engage a project advisory board of representatives from academia, universities, state and federal agencies, industry, and growers. While we are doing the science, the advisory board will provide guidance on both the technical and practical aspects of the project.”

Project team members:

—Kranthi Mandadi, Texas A&M AgriLife Research.     

—Mike Irey, Southern Gardens Citrus, Florida.    

—Choaa El-Mohtar, University of Florida Institute of Food and Agricultural Sciences, Citrus Research and Education Center.

—Ray Yokomi, USDA-Agricultural Research Service, Parlier, California.

—Ute Albrecht, University of Florida IFAS Southwest Florida Research and Education Center.   

—Veronica Ancona, Texas A&M University-Kingsville Citrus Center.

—Freddy Ibanez-Carrasco, Texas A&M AgriLife Research, Department of Entomology, Weslaco.

—Sonia Irigoyen, AgriLife Research, Texas A&M AgriLife Research and Extension Center at Weslaco.

—Ariel Singerman, University of Florida IFAS Citrus Research and Education Center.

—Jinha Jung, Purdue University, Indiana.     

—Juan Enciso, Texas A&M AgriLife Research, Department of Biological and Agricultural Engineering, Weslaco.

—Samuel Zapata, Texas A&M AgriLife Extension, Department of Agricultural Economics, Weslaco.

—Olufemi Alabi, Texas A&M AgriLife Extension, Department of Plant Pathology and Microbiology, Weslaco.

—Sonia Rios, University of California Cooperative Extension, Riverside and San Diego counties.

—Ben Faber, University of California Cooperative Extension, Ventura, Santa Barbara and San Luis Obispo counties.

—Greg Douhan, University of California Cooperative Extension, Tulare, Fresno and Madera counties.

 

Officials release new weevil to battle yellow starthistle

In April 2021, scientists released weevils from the Mediterranean region of Europe at the Bureau of Land Management Magnolia Ranch day-use area in El Dorado County to join the battle against yellow starthistle. Yellow starthistle rosette weevil is a newly approved natural enemy of yellow starthistle, which was introduced in California more than 150 years ago and, with no natural enemies in its new location, became one of the state's most harmful weeds, infesting nearly 15 million acres.

In California, yellow starthistle can grow to shoulder height, forming massive, thorny patches that block hiking trails, crowd out native plants and present a wildfire danger. The plant is toxic to horses and its flowers are encircled by inch-long sharp spines that can pierce the eyes of grazing animals.

UC Cooperative Extension has worked for decades with landowners to manage yellow starthistle-invested land.

“Over the years, we have developed effective control strategies for yellow starthistle – including well-timed mowing, grazing, hand pulling, burning, cultivation and herbicide application,” said Scott Oneto, UC Cooperative Extension natural resources advisor in El Dorado County. “But these treatments are rarely implemented on a scale large enough to combat our enormous starthistle infestations.”

Efforts to introduce natural enemies from yellow starthistle's home range began in the 1960s. Several species were released to attack starthistle flower heads. These insects lay eggs, hatch and feed on developing seeds, reducing seed production.

“Although the flower head insects sometimes attack high proportions of flowers, yellow starthistle is a very prolific seed producer with an individual plant producing as much as 100,000 seeds,” Oneto said. “Even if the insects reduce seed production by 50%, that still leaves a lot of seeds.”

The newly introduced yellow starthistle rosette weevil, first collected in Turkey as a prospective biocontrol agent in 1984, attacks the plant at the base. Since 2001, United States Department of Agriculture research entomologist Lincoln Smith has studied the insect at the USDA Agricultural Research Service laboratory in Albany.

“Larvae of the weevil develop and feed inside the root crown during spring, adults emerge in June, and then they spend the rest of the year hiding,” Smith said. “There is only one generation per year, so populations will grow slowly, which will gradually reduce yellow starthistle populations.”

The weevil presents no risk to other plants in California except bachelor's buttons, which is an introduced plant from Europe, but not considered a noxious weed. Successful biological control with the weevil is expected to reduce yellow starthistle, but not completely eliminate the weed.

This was the second release of the beneficial insect in North America. The first release occurred in Solano County in April 2020.

Bureau of Land Management and University of California Cooperative Extension researchers will be monitoring the Magnolia Ranch site intensively over the next several years to determine the rate of rosette weevil reproduction and efficacy at feeding on yellow starthistle.

For more information, see A New Warrior Released in the Battle to Control Yellow Starthistle by Scott Oneto on the UC Weed Science blog.

Grow it: Gardening tips and resources

Gardening is fun…and it's an important activity. What we grow in school, home and community gardens can improve our health, and the health of our families and communities. What we grow can increase the resiliency of food systems in our communities. And what we grow, ultimately, can connect us more closely with the earth that sustains us. There are valuable lessons in gardening…too many to list here.

Even if you live in a small apartment, you can grow food. If you have a yard, you can grow quite a lot of food. View the transformation of a front yard in an urban area…from lawn to lush, productive food garden in only 60 days. You'll love the progression photos, and the simple explanation about how the garden came together.

Need more inspiration? Roger Doiron, founder of SeedMoney, talks about his (subversive) garden plot in this remarkable TedX talk. Roger created and led the social media campaign that called for a garden at the White House. This campaign ultimately led First Lady Michelle Obama to plant a vegetable garden at the White House. (And it may have also inspired the People's Garden at the USDA, which broke ground on Abraham Lincoln's birthday 10 years ago. Lincoln referred to the USDA as the “People's Department,” so it makes sense that the USDA would refer to its garden as the “People's Garden.”)

Need practical advice? The UC Master Gardener program has more than 5,000 certified volunteers ready to assist if you live in California. UC has also created a California Garden Web portal that provides a treasure trove of gardening resources for all parts of the state. It's not too early to begin planning your Fall garden, and you'll find information about that, too.

If you're interested in school gardens, read this brief history, written by UC ANR's UC Food Observer.

Happy gardening!

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