- Author: Ben Faber
An Automated Delivery System for Therapeutic Materials to Treat HLB Infected Citrus
Ozgur Batuman1 and Louise Ferguson2
¹Southwest Florida Research and Education Center, University of Florida, Immokalee, FL; 2UC Davis, Department of Plant Sciences, University of California Davis, Davis CA
Why is this research needed?
In 2005, a disease called Huanglongbing (HLB, citrus greening, was identified in Florida's commercial citrus groves. The disease is caused by a bacterium that affects all citrus cultivars by disrupting the flow of nutrients from the source of production, to the site of use, causing tree decline. HLB weakens the root system, increases early fruit and leaf drop, lowers tree productivity and fruit quality and ultimately kills the tree. The disease has spread to all the major production regions in Florida. Economic losses have exceeded more than $4 billion dollars. Currently, more than 95% of Florida's trees are infected. There is currently no cure for the disease.
Efforts to control HLB have been unsuccessful as the bacterium cannot be cultured, literally grown, in a petri dish, and once in the plant it proliferates within the citrus phloem. Phloem is the system that transports sugars from their site of production, the leaves, to plant parts that use sugars, the roots or flowers.Phloem transport is generally downward but can be upward as well.
Once the HLB bacterium is in a tree's phloem it has the potential to infect the entire tree. It is exceedingly difficult to introduce any control agent into the phloem with the conventional control methods of foliar spraying or soil drenching.
Thus far, no treatment preventing HLB infection, or controlling the bacterium once within the tree, has been developed. Potential chemicals are being investigated, but in order to test them, direct or indirect phloem delivery, where the bacterium proliferates, is needed. Therefore, an effective method of delivering an effective volume of theraputics into the phloem is needed to evaluate potential treatments.
What is the focus of this project?
Our project focuses on developing a method of delivering therapeutic liquid materials, bactericides, microbial metabolites, RNAi, or biologicals, into the citrus vascular tissues, both the xylem which conducts water and nutrients upward from the roots and the phloem, which conducts sugars and other metabolic products downward from the leaves. We are investigating diffusion, trunk punctures with a surrounding liquid reservoir for passive uptake and infusion, low pressure active injections. We are focusing on these methods as foliar sprays and root drenches have not been successful phloem delivery methods.
Who will be doing the research?
The project is led by plant pathologist Dr. Ozgur Batuman with colleagues at the Southwest Florida Research and Education Center (SWFREC) at University of Florida in Immokalee. This four-year project will also study the citrus vascular system with a multidisciplinary research team including UF Plant Pathologists Drs. Nabil Killiny and Amit Levy at Lake Alfred, SWFREC UF Plant Physiologist Ute Albrecht, Citrus Horticulturist Fernando Alferez, Precision Ag. Engineer Yiannis Ampatzidis, Agricultural and Natural Resources Economist Tara Wade, University of California-Davis Extension Specialist Louise Ferguson and Texas A&M-Kingsville Citrus Center Plant Pathologist Veronica Ancona as well as number of graduate students, postdocs, and Florida, Texas and California citrus industry members.
How will this research be done?
Our earlier research involving comparisons of delivery methods including foliar sprays, soil drenching and trunk injection determined Needle-Assisted Trunk Infusion (NATI) was the best potential delivery method (Figure.1). In initial experiments, using NATI, 1 ml of rhodamine (1%) dye was injected into the trunks of one-year-old citrus seedlings. A visible red color, indicative of rhodamine uptake and movement, was detected in the upper-most leaves within 30-60 min and an increase in color intensity was observed within 24 hours. Similar results were observed in two-year-old grafted Valencia plants within 48 hours. If the NATI delivery method can be automated, large numbers of trees could be treated quickly. Once the delivery method has been developed, implementation will be tested with potential treatments developed within other research projects.
Our proposed automated delivery would consist of a robotic arm with several modules at the end of the arm, installed on an ATV or tractor. One module with needles would grip and puncture the trunk, a second module would wrap a reservoir around the trunk below the punctures and third module would fill the reservoir. (Figure 2). Hopefully, a robotic arm plus automated system will be inexpensive enough for growers to purchase and simple enough to use.
Another approach is disease prevention; application pf prophylactic chemicals that prevent infection. In this scenario our system would be used treat healthy young trees with bactericides or boost their immune system. When infected by the ACP the bacterium would either be killed or suppressed, perhaps below the level that harms tree growth and productivity. This option is analogous to the vaccinations that prevent diseases in humans and animals.
What are the greatest challenges and opportunities.
The greatest challenge is successful phloem delivery. The greatest opportunity is that, if successful, we will have developed a method that will allow much more precise deliver of theraputics to citrus trees. For example, if an effective phloem delivery method is developed, it could be used to control insects that feed on citrus plant parts. Or, it could be used to deliver growth regulators, perhaps nutrients and carbohydrates, to roots and fruits to increase growth, development and fruit quality; much like an intravenous injection functions in an animal.
Among the questions we hope to investigate are:
- When, what kind of, and what amount of therapeutics can be applied by NATI?
- At what frequency?
- What type of citrus tree: cultivar, age, infected, healthy is the best for treatment by NATI?
- Can we kill the bacterium? How and when to assess a change in bacteria titer after treatment?
- When will become available and be economically feasible for growers?
Figure 1. Distribution of rhodamine (red dye; 1%) applied by NATI in various tissues (left) of grafted and non-grafted young citrus plants grown in the greenhouse (right). Photos taken 2 weeks after the treatments. Treatments and tissues observed are indicated. Yo = year-old.
Figure 2. Projected automated delivery system (ADS); an ATV with extendable arm with NATI and the cover placement systems on the arm guided onto the tree trunk (upper panel), and closeup of NATI and cover placement system (panel below).
Acknowledgement
The United States Department of Agriculture National Institute of Food and Agriculture (USDA-NIFA) Grant # 2019-70016-29096.
For more information, please visit this project's dedicated website:
https://swfrec.ifas.ufl.edu/programs/citrus-path/automated-delivery/
- Author: Ben Faber
The calls have come in. We've gone from cool to hot and Dry Root Rot of Lemon has struck, It's shocking how fast the trees go down.
Dry Root Rot has menaced growers in Ventura County for many years. In the ‘50's and ‘60's it seemed most prevalent on older orange trees. A few years after the wet winter of 1968-69, dry root rot became an increasing problem among citrus trees of all ages. At that time, most of the damaged trees were on sweet rootstock (susceptible to Phytophthora), and growing in fine-textured soils or soils with poor drainage. A few years after another wet winter/spring (of 1983), dry root rot again reared its ugly head, but this time predominately on young lemons.
The disease is caused by the fungus, Fusarium solani. This fungus is most likely present in all citrus soils in California. It is a weak pathogen in that by itself it will not attack a healthy tree. However, experiments conducted in the early 1980's by Dr. Gary Bender, showed that when seedlings were girdled, root invasion occurred. In the field, the fungus can infect trees once gophers have girdled the roots or crown. A Phytophthora infection will also predispose trees to Fusarium, as will asphyxiation. Therefore, the mere presence of the fungus in the orchard soil will not lead to the disease.
Description
Fusarium is a soil borne fungus that invades the root system. Once infected, the entire root will turn reddish-purple to grayish-black. This is in contrast to a Phytophthora infection which, in many cases, will attack only the feeder roots, but when larger roots are infected, only the inner bark is decayed and it does not discolor the wood. In addition, when observing the cross section of a dry root rot infected trunk, a grayishbrown discoloration in the wood tissue can be observed.
Dry root rot is a root disease, but symptoms of the root decline are seen above ground. They are similar to any of the root and crown disorders such as Phytophthora root rot, oak root rot fungus (Armillaria) and gophers. The trees lack vigor, leaves begin to turn yellow and eventually drop (especially in hot weather) causing twig dieback. Finally, the foliage will become so sparse that one will be able to see through the canopy of the tree. A period of two to three years may pass from the time of invasion until noticeable wilt. Many times, the tree will collapse in the summer, after a period of prolonged heat. In the case of dry root rot, the collapse is so rapid that the tree dies with all the leaves still on the tree. When looking for symptoms of dry root rot, keep an eye out for symptoms of other maladies as well — Phytophthora, oak root rot fungus and gophers being the most prevalent.
As mentioned previously, in order for Fusarium to infect a tree, there must be a predisposing factor such as girdling from gopher feeding. However, since many trees collapse from dry root rot without any apparent predisposing factor, there are obviously other factors which we have yet to identify. Therefore, in 1998, a grower survey was developed, along with intensive soil and leaf sampling, to attempt to identify as many new predisposing factors as possible. They might be elements in the soil, either deficiencies or excesses, or specific cultural practices such as irrigation patterns or fertilizer practices. Twenty orchards were identified from which 20 soil and 20 leaf samples were taken in diseased areas and another 20 soil and 20 leaf samples were taken from adjacent healthy areas. The owners or managers of the properties were given a questionnaire to complete regarding a variety of cultural operations. The objective was to identify those factors that would correlate well to trees becoming infected with dry root rot.
Survey Results
Soil analysis - The following laboratory procedures were conducted to see if there was any correlation between the disease and either deficiencies or toxicities of these elements or
conditions: sodium, boron, salt level, pH and soil type (sand, loam, clay). For these elements or conditions, no correlation was found. It would appear that for our sampling sites, these conditions, whether favorable or not (toxic or deficient), did not play a major role in predisposing the tree to dry root rot.
Leaf analysis - The following elements were analyzed for their concentration within the leaf: nitrogen, potassium, phosphate, manganese, magnesium and zinc. Of these, three correlations were found. Zinc and manganese levels were substantially higher in diseased trees. The third correlation showed a potassium deficiency in diseased trees. However, we do not believe that dry root rot is caused by elevated levels of zinc or manganese, or by potassium deficiency, but rather are a result of the disease. Unfortunately, it seems that we have still not identified any elements in leaf analysis that truly correlates and points to a predisposing factor for disease development.
Control Measures – What Works and What Does Not
Early experiments conducted by Menge, Ohr and Sakovich showed that the following circumstances or operations do not influence the incidence of this disease: fungicidal treatments, wounding the tap root at time of planting, sandy versus clay textured soils, spring versus fall planting and soil mounding.
- In choosing your nursery tree, the choice of rootstock is not important in that, as far as we know, all rootstocks are susceptible to this disease. However, since Phytophthora is a major component in dry root rot development, choosing a rootstock like sweet orange would certainly put those trees in a high risk category. We recommend that growers use Phytophthora resistant rootstocks like C35 or Citrumelo.
Phytophthora. Publications written in the 1970's, and again noted by our survey, showed that Phytophthora is a major culprit in the dry root rot complex. To control dry root rot, it is essential that the Phytophthora, when present, be controlled. This can be accomplished by fungicidal treatments, and by the proper application and timing of irrigation water. Overwatering creates a favorable environment for the multiplication of the Phytophthora fungus.
Gophers. It is well known that gopher damage provides entry points for Fusarium. Controlling gophers is an important factor in reducing the potential of infection by Fusarium.
Control
We presently have no direct control for dry root rot. To control the disease, we must control the predisposing factors such as gophers, Phytophthora, poor drainage and over-watering. If the predisposing factor(s) cannot be identified for a given diseased orchard, it will indeed be difficult to control the disease. Two things are certain though: 1.) There are no chemicals to date which will control this disease; and 2.) Presently, there are no rootstocks resistant to the disease.
Listen to Akif Eskalen tell the Dry Root Rot story
https://www.youtube.com/watch?v=K2fyBcC1HXk&feature=youtu.be
- Author: Ben Faber
What Can Happen With Too Much Rain?
Rain is wonderful stuff. If it comes and washes the accumulated salts of the last several years out of the root zones of citrus and avocado, that's a good thing. But what happens if there is a little too much of the good stuff? In the winter of 2005, Venture got over 40” of rain, which is 200% of what is normal. The last time big rains occurred prior to that was in the winter of 1997-98. That year the rains were evenly spaced on almost a weekly basis through the winter and into the late spring and over 50" fell. That year we had major problems with both citrus and avocados collapsing from asphyxiation. The same occurred in 2005, but not so pronounced.
This April we have had a lot more rain than we normally see and in some young trees with poorly developed root systems, we have seen some collapse from asphyxiation. Avocados tend to be more susceptible than citrus, and some rootstocks more than others.
Asphyxiation is a physiological problem that may affect certain branches, whole limbs or the entire tree. Leaves wilt and may fall, the fruit withers and drops and the branches die back to a greater or lesser extent. The condition develops so rapidly that it may be regarded as a form of collapse. Usually, the larger stems and branches remain alive, and after a time, vigorous new growth is put out so that the tree tends to recover. Young trees can be harder hit, but sunburn damage from lack of leaves may be more of a problem.
Asphyxiation is related to the air and water conditions of the soil. The trouble appears mainly in fine-textured or shallow soils with impervious sub-soils. In 1997-98, this even occurred on slopes with normally good drainage because the rains were so frequent. When such soils are over-irrigated or wetted by rains, the water displaces the soil oxygen. The smaller roots die when deprived of oxygen. When the stress of water shortage develops, the impaired roots are unable to supply water to the leaves rapidly enough and the tree collapses. The condition is accentuated when rainy weather is followed by winds or warm conditions. These are exactly the conditions we saw in the last weeks of April and beginning of May - wet weather and then 90 degree heat. Boom. hence some of the problems in young orchards on heavier soils.
It doesn't take standing water to have asphyxiation occur.
Canopy treatment in less severe instances of asphyxiation consists of cutting back the dead branches to live wood. If leaf drop has been excessive, the tree should be whitewashed to prevent sunburn. Fruit, if mature should be harvested as soon as possible to prevent loss. In the case of young trees, less than two years of age, recovery sometimes does not occur, and replanting should be considered if vigorous regrowth does not occur by July. As soon as defoliation is evident, whitewashing should be done to protect them to give them a chance for recovery.
Asphyxiation can be reduced by proper planting and grading. If an impervious layer is identified, it should be ripped prior to planting. The field should be graded so that water has somewhere to run off the field during high rainfall years. Heavier soils might require planting on berms or mounds so that the crown roots have a better chance of being aerated.
Hindsight is always great. Post-plant, if an impervious layer can be identified and is shallow enough to break through, ripping alongside the tree or drilling 4-6 inch post holes at the corners of the tree canopy can improve drainage. It is important that the ripper blade or auger gets below the impervious layer for this technique to be effective. If there is a thick layer of mulch reducing soil evaporation, pulling it back to allow the sun to help dry it out faster will help. It's not a lot of work with small trees, but big time work if it's big trees with thick mulch.
Asphyxiated tree that has been whitewashed
- Author: Ben Faber
The University of California Cooperative Extension San Diego County presents:
The Climate Action & Agriculture Symposium Webinar:
Soil Health
The health and safety of our communities is a priority. Due to Coronavirus disease 2019(COVID-19), the UC Cooperative Extension is hosting a FREE webinar in lieu of the day-long event as previously announced.
Thursday, May 21,2020
1:00pm–3:30pm|FREE: Registration Required
Description:
The Climate Action and Agriculture Symposium focuses on the policies, research, programs, strategies and resources needed to support sustainable agriculture and the reduction of greenhouse gas emissions via the unique agricultural environments and industry in San Diego County.This symposium is intended to bring together agricultural producers, policy makers, government agencies, researchers, educators, consultants and other public and private agricultural service providers.
Presentations:
Climate Trends & Impacts to Agriculture
Tapan Pathak, Ph.D., UCANR Specialist,Climate Adaptation in Agriculture
Integrated Soil Management
Joji Muramoto, Ph.D., UC ANR Specialist, Organic Production
Mulching and Cover Crop Strategies: Avocados and Other Orchard Crops
Ben Faber, Ph.D., UCCE Farm Advisor
Cover Crop Strategies: Vegetables
Oli Bachie, Ph.D., UCCE County Director-Imperial & Farm Advisor
Spadra Farm Healthy Soils Program Research and Demonstration Project
TBA- Agriculture & Plant Science Departments, California State Polytechnic University, Pomona
Opening Remarks by Hannah Gbeh,
Executive Director, San Diego County Farm Bureau
Lightening Talk Session:
Brief updates on recent and current climate and agriculture projects.
TALKS INCLUDE:
CDFA/UC ANR Healthy Soils Program, County of San Diego Compost & Mulch Market Study, Climate Science Alliance 2020 Climate Change Consortium for Specialty Crops, San Diego Food System Alliance Food Vision 2030,and more.
Jan Gonzales(jggonzales@ucanr.edu)
- Author: Ben Faber
The 2020 HSP Incentives Program will award up to $22 million in grants to farmers and ranchers for projects that implement agricultural management practices to improve soil health and reduce greenhouse gas (GHG) emissions. As of Thursday, May 7, 2020, CDFA has received 459 applications requesting a total of $30.39 million in funding. Of these, 273 applications requesting a total of $18.8 million have been preliminarily selected for awards based on completed technical reviews.
CDFA anticipates completing technical reviews for applications that have already been submitted and selecting projects for awards totaling $22 million by Friday, May 15, 2020. It is anticipated that the application submission deadline would be closed by 5 p.m. on Friday, May 15, 2020. CDFA cannot close the submission period prior to selecting for award $22 million in grants to be consistent with language on page 3 of the Request for Grant Applications: “The application submission period will be on a rolling basis, starting February 27, 2020 and continuing until June 26, 2020 or until available funds are expended, whichever is earlier.”
At this time, CDFA recommends potential applicants to reconsider submitting additional applications as they may not be funded in this year's first-come, first-serve process. We thank you for your enthusiastic participation and interest in the 2020 HSP Incentives Program.
Sincerely,
CDFA-Office of Environmental Farming and Innovation (OEFI) HSP Team
